{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1030073": {"GENOTYPE": "control"}}, {"GSM1030073": {"GENOTYPE": "control"}}, {"GSM1030073": {"GENOTYPE": "control"}}, {"GSM1030076": {"GENOTYPE": "Estrogen Receptor alpha knock-out"}}, {"GSM1030076": {"GENOTYPE": "Estrogen Receptor alpha knock-out"}}, {"GSM1030076": {"GENOTYPE": "Estrogen Receptor alpha knock-out"}}], "id": 7069, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Estrogens are well known steroid hormones necessary to maintain bone health. In addition, mechanical loading, which estrogen signaling...", "geo_gse_id": "E-GEOD-41997", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["bone", "estrogen", "osteoclast", "osteocyte", "trabecular bone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-dmp1-gfp-sorted-osteocytes", "geo_id_plat": "E-GEOD-41997_A-AFFY-45", "name": "Expression data from Dmp1-GFP sorted osteocytes", "created": "Nov.12, 2014", "summary": "Estrogens are well known steroid hormones necessary to maintain bone health. In addition, mechanical loading, which estrogen signaling may intersect with the Wnt/\u03b2-catenin pathway, is also essential for bone health. As osteocytes are known as the major mechanosensory cells embedded in mineralized bone matrix, osteocyte ER\u03b1 deletion mice (ER\u03b1\u0394Ocy/\u0394Ocy) were generated by mating ER\u03b1 floxed mice with Dmp1-Cre mice to determine functions of ER\u03b1 in osteocytes. Trabecular bone mineral density of female, but not male ER\u03b1\u0394Ocy/\u0394Ocy mice was significantly decreased. Bone formation parameters in ER\u03b1\u0394Ocy/\u0394Ocy were significantly decreased while osteoclast parameters were unchanged. This suggests that ER\u03b1 in osteocytes exerts osteoprotective function by positively controlling bone formation. To identify potential targets of ER\u03b1, gene array analysis of Dmp1-GFP osteocytes FACS sorted from ER\u03b1\u0394Ocy/\u0394Ocy and control mice was performed. Expression of Mdk and Sostdc1, both known inhibitors of Wnt, were significantly increased without alteration of the mature osteocyte marker Sost or \u03b2-catenin. Hindlimb unloading exacerbated the trabecular bone loss, but surprisingly cortical bone was resistant. These studies show that ER\u03b1 in osteocytes has osteoprotective effects in trabecular bone through regulating expression of Wnt antagonists, but conversely plays a negative role in cortical bone loss due to unloading. Wild type and osteocyte-specific Estrogen Receptor alpha knock-out mice were generated. The number of both genotypes of mice was three. Calvarial osteocytes of both genotypes harboring Dmp1-GFP were extracted by sequential enzymatic digestion, followed by FACS Aria sorting and total RNAs were purified for Affymetix GeneChip microarray analysis without pooling.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41997", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41997/samples/"}