{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2482, "factors": [{"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}, {"GSM102740": {"SAMPLE COLLECTION": "after-exercise"}}, {"GSM1027400": {"SAMPLE COLLECTION": "before-exercise"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We tested the hypothesis on the mechanisms responsible for the early control of NK cell function by identifying a discrete set of genes...", "pubmed_id": 23288554, "geo_gse_id": "E-GEOD-41914", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 24, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "effects-of-exercise-on-gene-expression-level-in-2", "geo_id_plat": "E-GEOD-41914_A-AFFY-44", "name": "Effects of exercise on gene expression level in human NK Cells", "created": "Jul.12, 2014", "summary": "We tested the hypothesis on the mechanisms responsible for the early control of NK cell function by identifying a discrete set of genes in circulating NK cells that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in NK cells. Twelve healthy men (20-29 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 77% of VO2max. A baseline blood sample was taken before the and immediately after the exercise.  NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS\u00ae Pro Separator). Total RNA was extracted using TRIzol\u00ae.  For this study we used Affymetrix plus 2  (total of 24 chips).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41914", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41914/samples/"}