{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1025390": {"VARIATION": "Zbtb7a Flox/+ Mx1-Cre+ (LRF heterozygous)"}}, {"GSM1025390": {"VARIATION": "Zbtb7a Flox/+ Mx1-Cre+ (LRF heterozygous)"}}, {"GSM1025392": {"VARIATION": "Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)"}}, {"GSM1025392": {"VARIATION": "Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)"}}, {"GSM1025392": {"VARIATION": "Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)"}}], "id": 7059, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "LRF, which is encoded by the ZBTB7A gene and formerly known as POKEMON (POK erythroid myeloid ontogenic factor), was originally...", "geo_gse_id": "E-GEOD-41839", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 5, "tags": ["b-cell lymphoma", "cell", "finger", "leukemia", "lymphoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-control-and-lrf-leukemialymph", "geo_id_plat": "E-GEOD-41839_A-AFFY-45", "name": "Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs", "created": "Nov.12, 2014", "summary": "LRF, which is encoded by the ZBTB7A gene and formerly known as POKEMON (POK erythroid myeloid ontogenic factor), was originally identified as a PLZF (promyelocytic leukemia zinc finger) homologue interacting with BCL6 (B-cell lymphoma 6). LRF is a transcription factor that is broadly expressed in hematopoietic lineage cells, but its expression is particularly high in erythroblasts and germinal center (GC) B-cells. The goal of this study is to assess the effect of LRF loss on the LT-HSC transcriptome. Nine days after injection of adult mice with polyinosinic polycytidylic acid (pIpc) to activate Cre, total RNAs were isolated from double-sorted LT-HSCs from LRF Flox/+ Mx1-Cre+ and LRF Flox/Flox Mx1-Cre+ mice and processed for microarray analysis. We performed gene expression microarray analysis of FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-) to assess the effect of Lrf loss on the LT-HSC transcriptome. Zbtb7a Flox/+ Mx1-Cre+ mice were used as a control to normalize the potential effects of Cre recombinase. LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41839", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41839/samples/"}