{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 2707, "factors": [{"GSM1025054": {"compound": "casiopeina II-gly"}}, {"GSM1025054": {"compound": "casiopeina II-gly"}}, {"GSM1025054": {"compound": "casiopeina II-gly"}}, {"GSM102505": {"compound": "control"}}, {"GSM102505": {"compound": "control"}}, {"GSM102505": {"compound": "control"}}], "pop_total": 0, "platform": 4, "summary_wrapped": "Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer...", "pubmed_id": 23382936, "geo_gse_id": "E-GEOD-41827", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cancer", "cell", "genome", "line", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-hela-cells-aft", "geo_id_plat": "E-GEOD-41827_A-AFFY-44", "name": "Transcription profiling by array of HeLa cells after treatment with casiopeina II-gly", "created": "Jul.12, 2014", "summary": "Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis -in a process mediated by reactive oxygen species- for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions; before establishing settings and parameters needed for their wide clinical application. Microarrays were used to determine the expression profile from HeLa cells in order to propose a model for the role played by intrinsic apoptosis triggered by the oxidative stress caused by Cas-II-gly. The cervix-uterine cell line HeLa\u00a0\u00a0was\u00a0maintained at 37C in 5% CO2\u00a0 under sterile conditions in Dulbecco's modified Eagle medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma). Cells were treated with Cas II-gly in 96-well microplates by 6 h.\u00a0\u00a0HeLa cells whole genome gene expression experiments (triplicates for cases/controls) were performed in total mRNA extracted under the GPL570 protocol.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41827", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41827/samples/"}