Dataset: Transcription profiling by array of HeLa cells after treatment with casiopeina II-gly
Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer...
Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis -in a process mediated by reactive oxygen species- for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions; before establishing settings and parameters needed for their wide clinical application. Microarrays were used to determine the expression profile from HeLa cells in order to propose a model for the role played by intrinsic apoptosis triggered by the oxidative stress caused by Cas-II-gly. The cervix-uterine cell line HeLa was maintained at 37C in 5% CO2 under sterile conditions in Dulbecco's modified Eagle medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma). Cells were treated with Cas II-gly in 96-well microplates by 6 h. HeLa cells whole genome gene expression experiments (triplicates for cases/controls) were performed in total mRNA extracted under the GPL570 protocol.
- Species:
- human
- Samples:
- 6
- Source:
- E-GEOD-41827
- PubMed:
- 23382936
- Updated:
- Dec.12, 2014
- Registered:
- Jul.12, 2014
Sample | compound |
---|---|
GSM1025054 | casiopeina II-gly |
GSM1025054 | casiopeina II-gly |
GSM1025054 | casiopeina II-gly |
GSM102505 | control |
GSM102505 | control |
GSM102505 | control |