{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 4600, "factors": [{"GSM1023406": {"TREATMENT": "pre-miR-27a"}}, {"GSM1023407": {"TREATMENT": "pre-miR-control"}}, {"GSM1023408": {"TREATMENT": "anti-miR-27a"}}, {"GSM1023409": {"TREATMENT": "anti-miR-control"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Gene expression profiling was carried out in Huh-7.5 cells in which miR-27a was over- or under-expressed. Transfection of cells with pre-...", "pubmed_id": 23449803, "geo_gse_id": "E-GEOD-41737", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["cytoskeleton", "hepatitis", "hepatitis b", "hepatitis c", "lipid", "liver"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "microrna-27a-regulates-lipid-metabolism-and-inhibi", "geo_id_plat": "E-GEOD-41737_A-AFFY-44", "name": "MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells", "created": "Sep.19, 2014", "summary": "Gene expression profiling was carried out in Huh-7.5 cells in which miR-27a was over- or under-expressed. Transfection of cells with pre-miR-27a and pre-miR-control, or anti-miR-27a and anti-miR-control enabled down- and up-regulated genes to be determined, respectively. Replication and infectivity of the lipotrophic hepatitis C virus (HCV) is regulated by cellular lipid status. Among differentially expressed micro (mi)RNAs, we found that miR-27a was preferentially expressed in HCV-infected compared with hepatitis B virus (HBV)-infected liver. Gene expression profiling of Huh-7.5 cells showed that miR-27a regulates lipid metabolism by targeting the lipid synthetic transcriptional factor, RXR\u03b1, and the lipid transporter, ABCA1 Carrying out a Target Scan (Release 5.2) of miR-27a predicted 921 candidate target genes, and functional gene ontology enrichment analysis of these genes by MetaCore (Thomson Reuters, NY) showed that miR-27a could target the signaling pathways of cytoskeleton remodeling and lipid metabolism .   To examine whether these signaling pathways were regulated by miR-27a, gene expression profiling was carried out in Huh-7.5 cells in which miR-27a was over- or under-expressed. Transfection of cells with pre-miR-27a and pre-miR-control, or anti-miR-27a and anti-miR-control enabled down- and up-regulated genes to be determined, respectively. Huh-7.5 cells with miR-27a over- or under-expressed", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41737", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41737/samples/"}