{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1018194": {"CELL TYPE": "IL10+CD25+CD4+ T-cells"}}, {"GSM1018194": {"CELL TYPE": "IL10+CD25+CD4+ T-cells"}}, {"GSM1018196": {"CELL TYPE": "IL10-CD25+CD4+ T-cells"}}, {"GSM1018196": {"CELL TYPE": "IL10-CD25+CD4+ T-cells"}}], "id": 7045, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The ability of Treg-cells to produce interleukin-10 (IL-10) is important for the limitation of  inflammation at environmental interfaces...", "geo_gse_id": "E-GEOD-41492", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["colon", "interleukin", "interleukin-10", "lung", "lymph", "lymph node", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-41492_A-AFFY-45", "slug": "sequential-induction-of-effector-function-tissue-m", "geo_gds_id": "", "name": "Sequential induction of effector function, tissue migration and cell death during polyclonal activation of regulatory T-cells", "created": "Nov.12, 2014", "summary": "The ability of Treg-cells to produce interleukin-10 (IL-10) is important for the limitation of  inflammation at environmental interfaces like colon or lung. Under steady state conditions, however,  only few Treg-cells produce IL-10 ex vivo. To investigate the origin and fate of IL-10 producing Tregcells  we used a superagonistic mouse anti-mouse CD28 mAb (CD28SA) for polyclonal in vivo  stimulation of Treg-cells, which not only led to numeric expansion but also to a dramatic increase in IL-  10 production. IL-10 secreting Treg-cells strongly upregulated surface receptors associated with  suppressive function, and had higher but IL-10 independent, in vitro suppressive capacity than nonproducing  Treg-cells. Furthermore, polyclonally expanding Treg-cells shifted their migration receptor  pattern after activation from a lymph node-seeking to an inflammation-seeking phenotype, explaining  the preferential recruitment of IL-10 producers to sites of ongoing immune responses. Finally, we  observed that IL-10 producing Treg-cells from CD28SA stimulated mice were more apoptosis-prone in  vitro than their IL-10 negative counterparts. These findings support a model where prolonged activation  of Treg-cells results in terminal differentiation towards an IL-10 producing effector phenotype  associated with a limited lifespan, implicating built-in termination of immunosuppression. total samples analysed are 4", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41492", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41492/samples/"}