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Home › Dataset Library › Expression data in H1299 after retinoblastoma binding protein-2 (RBP2) knockdown

Dataset: Expression data in H1299 after retinoblastoma binding protein-2 (RBP2) knockdown

To identify genes whose expression was affected by RBP2, we performed gene expression microarray analysis in control and RBP2 depletion...

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To identify genes whose expression was affected by RBP2, we performed gene expression microarray analysis in control and RBP2 depletion human lung cancer cell line. Two individual shRNA (RBP2 KD1 and RBP2 KD2) were used to limit potential off-target effects. One scramble short haripin RNA (shRNA) and two individual shRNAs to the human RBP2 gene (RBP2 KD1 and RBP2 KD2) were used. Two replicates per group were used. H1299 cells were infected with lentivirus which carriy shRNAs for 3 days. RNA was isloated by Trizol reagent and analyzed by bioanalyzer. Microarray experiments was performed by the Affymetrix Gene Expression Service Laboratory at Academia Sinica.

Species:
human

Samples:
6

Source:
E-GEOD-41443

PubMed:
23722541

Updated:
Dec.12, 2014

Registered:
Jul.12, 2014


Factors: (via ArrayExpress)
Sample SHRNA GENOTYPE
GSM1017447 scramble control
GSM1017447 scramble control
GSM1017445 RBP2 KD2 RBP2 depletion
GSM1017445 RBP2 KD2 RBP2 depletion
GSM1017443 RBP2 KD1 RBP2 depletion
GSM1017443 RBP2 KD1 RBP2 depletion

Tags

  • cancer
  • cell
  • line
  • lung
  • lung cancer

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