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Home › Dataset Library › Expression data for HT29 cells treated with 5-aza-deoxy-cytidine [Affymetrix]

Dataset: Expression data for HT29 cells treated with 5-aza-deoxy-cytidine [Affymetrix]

The RNA samples from HT-29 (ATCC) colon cancer cell line were reverse transcribed into cDNAs and categorized in 3 groups with different...

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The RNA samples from HT-29 (ATCC) colon cancer cell line were reverse transcribed into cDNAs and categorized in 3 groups with different concentrations of 5-aza-deoxy-cytidine (5-Aza); in each group three replicative 150 mm cultures were treated with: 1) dimethyl sulfoxide (vehicle alone, 0 μM 5-Aza); 2) 5μM 5-Aza and 3) 10 μM 5-Aza; for five days We then used Affymetrix microarray platform to profile the gene expression of the 3 HT29 cell groups (3 replicates in each group) in order to search for differentially expressed genes The transcriptional response of HT-29 (ATCC) colon cancer cell line under 3 concentrations of 5-aza-deoxy-cytidine was investigated based on their RNA expression profiles

Species:
human

Samples:
9

Source:
E-GEOD-41364

PubMed:
23902433

Updated:
Dec.12, 2014

Registered:
Jul.11, 2014


Factors: (via ArrayExpress)
Sample TREATMENT
GSM1015549 10 μM of 5-Aza
GSM1015549 10 μM of 5-Aza
GSM1015549 10 μM of 5-Aza
GSM1015546 5 μM of 5-Aza
GSM1015546 5 μM of 5-Aza
GSM1015546 5 μM of 5-Aza
GSM1015543 0 μM of 5-Aza
GSM1015543 0 μM of 5-Aza
GSM1015543 0 μM of 5-Aza

Tags

  • cancer
  • cell
  • colon
  • colon cancer
  • line

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