{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1009613 1": {}}, {"GSM1009614 1": {}}], "id": 7032, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Box C/D-type small nucleolar RNAs (snoRNAs) are functional RNAs responsible for mediating 2\u2019-O-ribose methylation of ribosomal RNAs...", "geo_gse_id": "E-GEOD-41164", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 2, "tags": ["b-cell lymphoma", "cell", "intron", "lymphoma", "nucleolus", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-41164_A-AFFY-45", "slug": "expression-data-from-splenic-b-cells-isolated-from", "geo_gds_id": "", "name": "Expression data from splenic B-cells isolated from DmU50(HG-b) mice or wild-type C57BL/6J", "created": "Nov.12, 2014", "summary": "Box C/D-type small nucleolar RNAs (snoRNAs) are functional RNAs responsible for mediating 2\u2019-O-ribose methylation of ribosomal RNAs (rRNAs) within the nucleolus. Previously, in relation to a novel chromosomal translocation in a human B-cell lymphoma, we identified U50HG, a non-protein-coding gene that hosted a box C/D-type U50 snoRNA within its intron. To investigate the physiological importance of the U50 snoRNA and its involvement in tumorigenesis, we generated a mouse model deficient in mouse U50 (mU50) snoRNA expression without altering the expression of mouse mU50 host-gene, mU50HG-b. The established mU50 snoRNA-deficient mice showed a significant reduction of mU50 snoRNA expression and the corresponding target rRNA methylation in various organs. Lifelong phenotypic monitoring showed that the mU50-deficient mice looked almost normal without accelerated tumorigenicity; however, a notable difference was the propensity for anomalies in the lymphoid organs. B-cells were isolated from spleens of DmU50(HG-b) mice or wild-type C57BL/6J with antibody-conjugated magnetic beads system (Myltenyi Biotec). Total RNA was purified with QIAGEN RNeasy Micro kit. Affymetrix GeneChip\u00ae Microarrays (Mouse Expression 430 2.0 Array) were used.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41164", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-41164/samples/"}