{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM1006375": {"TREATMENT": "PDEF knockdown"}}, {"GSM1006375": {"TREATMENT": "PDEF knockdown"}}, {"GSM1006373": {"TREATMENT": "control"}}, {"GSM1006373": {"TREATMENT": "control"}}], "id": 2086, "pop_total": 0, "platform": 4, "summary_wrapped": "Microarray gene expression analysis was performed in MCF7 cells transduced with a non-specific shRNA or PDEF-targeting shRNA, and both...", "pubmed_id": 23764000, "geo_gse_id": "E-GEOD-40985", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["cell", "hormone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-profiles-induced-by-shrna-mediated", "geo_id_plat": "E-GEOD-40985_A-AFFY-44", "name": "Gene expression profiles induced by shRNA-mediated knockdown of PDEF in MCF7 mammary epithelial cell line", "created": "Jul.11, 2014", "summary": "Microarray gene expression analysis was performed in MCF7 cells transduced with a non-specific shRNA or PDEF-targeting shRNA, and both subjected to hormone depletion for 48 hours. Analyses of differentially expressed genes combined with gene ontology revealed a downregulation of cell cycle related-genes and an upregulation of apoptosis-related genes in PDEF knockdown cells. These target genes constitute potential effectors of the pro-survival role of PDEF. Four samples were harvested after lentiviral infection with either non-specific shRNA control or PDEF-targetting shRNA.  All samples were subjected to hormone depletion for 48 hours.  Each condition was performed in duplicate", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40985", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40985/samples/"}