ArrayExpress Uploaderarrayexpress_sidhumanEstrogen receptor positive breast cancerMCF-7controlEstrogen receptor positive breast cancerMCF-7controlEstrogen receptor positive breast cancerMCF-7controlEstrogen receptor positive breast cancerMCF-7ACSL4-inducedEstrogen receptor positive breast cancerMCF-7ACSL4-inducedEstrogen receptor positive breast cancerMCF-7ACSL4-inducedEstrogen receptor positive breast cancerMCF-7control-transfectedEstrogen receptor positive breast cancerMCF-7control-transfectedEstrogen receptor positive breast cancerMCF-7control-transfectedEstrogen receptor positive breast cancerMCF-7ACSL4-transfectedEstrogen receptor positive breast cancerMCF-7ACSL4-transfectedEstrogen receptor positive breast cancerMCF-7ACSL4-transfectedHER2-positive breast cancerSKBR3control-transfectedHER2-positive breast cancerSKBR3control-transfectedHER2-positive breast cancerSKBR3control-transfectedHER2-positive breast cancerSKBR3ACSL4-transfectedHER2-positive breast cancerSKBR3ACSL4-transfectedHER2-positive breast cancerSKBR3ACSL4-transfected458004ACSL4 expression appears to be inversely associated with steroid hormone and growth factor receptor expression in breast cancer and...E-GEOD-40968/profile/8773/arrayexpressuploader318basalbreastbreast cancercancerhormoneorfsteroid hormoneDec.12, 2014Falsethe-effect-of-acsl4-expression-on-overall-gene-expE-GEOD-40968_A-AFFY-44The effect of ACSL4 expression on overall gene expression in breast cancer cell linesSep.19, 2014ACSL4 expression appears to be inversely associated with steroid hormone and growth factor receptor expression in breast cancer and positively correlated with an aggressive breast cancer phenotype. Neither MCF-7 nor SKBr3 cells normally express ACSL4, and when manipulated to do so, develop basal-like characteristics, including increased proliferation, migration and anchorage independent growth. We used an Affymetrix array platform to assess changes in individual gene expression as a function of conditional and stable expression of ACSL4 in MCF-7 and SKBr3 cells. In experiment 1, MCF-7 Tet-ON cells were transfected with ACSL4 cDNA and induced to express ACSL4 by addition of 1ug/ml of doxycycline (A) or vehicle (C) for 72 hours. Three controls (C) and three doxycycline-induced (A) samples were evaluated for gene expression. In experiment 2, MCF-7 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A). In experiment 3, SKBr3 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40968http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40968/samples/