{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1006127": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "control"}}, {"GSM1006127": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "control"}}, {"GSM1006127": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "control"}}, {"GSM1006130": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "ACSL4-induced"}}, {"GSM1006130": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "ACSL4-induced"}}, {"GSM1006130": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "ACSL4-induced"}}, {"GSM1006133": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "control-transfected"}}, {"GSM1006133": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "control-transfected"}}, {"GSM1006133": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "control-transfected"}}, {"GSM1006136": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "ACSL4-transfected"}}, {"GSM1006136": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "ACSL4-transfected"}}, {"GSM1006136": {"DISEASE STATE": "Estrogen receptor positive breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "ACSL4-transfected"}}, {"GSM1006139": {"DISEASE STATE": "HER2-positive breast cancer", "CELL LINE": "SKBR3", "TREATMENT": "control-transfected"}}, {"GSM1006139": {"DISEASE STATE": "HER2-positive breast cancer", "CELL LINE": "SKBR3", "TREATMENT": "control-transfected"}}, {"GSM1006139": {"DISEASE STATE": "HER2-positive breast cancer", "CELL LINE": "SKBR3", "TREATMENT": "control-transfected"}}, {"GSM1006142": {"DISEASE STATE": "HER2-positive breast cancer", "CELL LINE": "SKBR3", "TREATMENT": "ACSL4-transfected"}}, {"GSM1006142": {"DISEASE STATE": "HER2-positive breast cancer", "CELL LINE": "SKBR3", "TREATMENT": "ACSL4-transfected"}}, {"GSM1006142": {"DISEASE STATE": "HER2-positive breast cancer", "CELL LINE": "SKBR3", "TREATMENT": "ACSL4-transfected"}}], "id": 4580, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "ACSL4 expression appears to be inversely associated with steroid hormone and growth factor receptor expression in breast cancer and...", "geo_gse_id": "E-GEOD-40968", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 18, "tags": ["basal", "breast", "breast cancer", "cancer", "hormone", "orf", "steroid hormone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-40968_A-AFFY-44", "slug": "the-effect-of-acsl4-expression-on-overall-gene-exp", "geo_gds_id": "", "name": "The effect of ACSL4 expression on overall gene expression in breast cancer cell lines", "created": "Sep.19, 2014", "summary": "ACSL4 expression appears to be inversely associated with steroid hormone and growth factor receptor expression in breast cancer and positively correlated with an aggressive breast cancer phenotype.  Neither MCF-7 nor SKBr3 cells normally express ACSL4, and when manipulated to do so, develop basal-like characteristics, including increased proliferation, migration and anchorage independent growth.  We used an Affymetrix array platform to assess changes in individual gene expression as a function of conditional and stable expression of ACSL4 in MCF-7 and SKBr3 cells. In experiment 1, MCF-7 Tet-ON cells were transfected with ACSL4 cDNA and induced to express ACSL4 by addition of 1ug/ml of doxycycline (A) or vehicle (C) for 72 hours.  Three controls (C) and three doxycycline-induced (A) samples were evaluated for gene expression.  In experiment 2, MCF-7 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A).  In experiment 3, SKBr3 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40968", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40968/samples/"}