ArrayExpress Uploader04578Subject 1salineSubject 1LPSSubject 2salineSubject 2LPSSubject 3salineSubject 3LPSSubject 4salineSubject 4LPSSubject 5salineSubject 5LPSSubject 6salineSubject 6LPSSubject 7salineSubject 7LPSarrayexpress_sid4Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or...22952057E-GEOD-40885/profile/8773/arrayexpressuploader214asthmachronic obstructive pulmonary diseasediseasegenomelipopolysaccharidelungpneumoniaDec.12, 2014Falsedata-expression-in-alveolar-macrophages-induced-byE-GEOD-40885_A-AFFY-44Data expression in alveolar macrophages induced by lipopolysaccharide in humansSep.19, 2014Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo. In seven healthy humans sterile saline was instilled into a lung segment by bronchoscope, followed by instillation of LPS into the contralateral lung. Six hours later a bilateral bronchoalveolar lavage was performed and whole-genome transcriptional profiling was done (Affymetrix HG-U133 Plus 2.0) on purified alveolar macrophages, comparing cells exposed to saline or LPS from the same individuals.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40885humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40885/samples/