{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM998968": {"GENOTYPE": "WT", "TREATMENT": "none"}}, {"GSM998968": {"GENOTYPE": "WT", "TREATMENT": "none"}}, {"GSM998968": {"GENOTYPE": "WT", "TREATMENT": "none"}}, {"GSM99897": {"GENOTYPE": "WT", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM99897": {"GENOTYPE": "WT", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM99897": {"GENOTYPE": "WT", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM99897": {"GENOTYPE": "WT", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM998975": {"GENOTYPE": "WT", "TREATMENT": "after 8 days of LCMV infection"}}, {"GSM998975": {"GENOTYPE": "WT", "TREATMENT": "after 8 days of LCMV infection"}}, {"GSM998975": {"GENOTYPE": "WT", "TREATMENT": "after 8 days of LCMV infection"}}, {"GSM998978": {"GENOTYPE": "WT", "TREATMENT": "after 8 days of LCMV infection and treated with IFNalpha for 90 minutes"}}, {"GSM998978": {"GENOTYPE": "WT", "TREATMENT": "after 8 days of LCMV infection and treated with IFNalpha for 90 minutes"}}, {"GSM998978": {"GENOTYPE": "WT", "TREATMENT": "after 8 days of LCMV infection and treated with IFNalpha for 90 minutes"}}, {"GSM99898": {"GENOTYPE": "STAT1 KO", "TREATMENT": "none"}}, {"GSM99898": {"GENOTYPE": "STAT1 KO", "TREATMENT": "none"}}, {"GSM99898": {"GENOTYPE": "STAT1 KO", "TREATMENT": "none"}}, {"GSM998984": {"GENOTYPE": "STAT1 KO", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM998984": {"GENOTYPE": "STAT1 KO", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM998984": {"GENOTYPE": "STAT1 KO", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM998987": {"GENOTYPE": "STAT4 KO", "TREATMENT": "none"}}, {"GSM998987": {"GENOTYPE": "STAT4 KO", "TREATMENT": "none"}}, {"GSM998987": {"GENOTYPE": "STAT4 KO", "TREATMENT": "none"}}, {"GSM998990": {"GENOTYPE": "STAT4 KO", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM998990": {"GENOTYPE": "STAT4 KO", "TREATMENT": "IFNalpha for 90 minutes"}}, {"GSM998990": {"GENOTYPE": "STAT4 KO", "TREATMENT": "IFNalpha for 90 minutes"}}], "id": 8286, "pop_total": 0, "platform": 7, "summary_wrapped": "Type 1 IFNs can conditionally activate all of the signal transducers and activators of transcription molecules (STATs), including STAT4....", "geo_gse_id": "E-GEOD-40666", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 25, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-40666_A-AFFY-23", "slug": "regulating-type-1-ifn-effects-in-cd8-t-cells-durin", "geo_gds_id": "", "name": "Regulating type 1 IFN effects in CD8 T cells during viral infections: changing STAT4 and STAT1 expression for function", "created": "Nov.23, 2014", "summary": "Type 1 IFNs can conditionally activate all of the signal transducers and activators of transcription molecules (STATs), including STAT4. The best-characterized signaling pathways use STAT1, however, and type 1 IFN inhibition of cell proliferation is STAT1 dependent. We report that type 1 IFNs can basally stimulate STAT1- and STAT4- dependent effects in CD8 T cells, but that CD8 T cells responding to infections of mice with lymphocytic choriomenigitis virus have elevated STAT4 and lower STAT1 expression with significant consequences for modifying the effects of type 1 IFN exposure. The phenotype was associated with preferential type 1 IFN activation of STAT4 as compared to STAT1. Stimulation through the TCR induced elevated STAT4 expression, and STAT4 was required for peak expansion of antigen-specific CD8 T cells, low STAT1 levels, and resistance to type 1 IFN-mediated inhibition of proliferation. Thus, a mechanism is discovered for regulating the consequences of type 1 IFN exposure in CD8 T cells, with STAT4 acting as a key molecule in driving optimal antigen-specific responses and overcoming STAT1-dependent inhibition of proliferation. CD8 T cells were purified from uninfected WT, STAT1-deficient and STAT4-deficient mice or from D8 LCMV-infected WT mice and either control treated or treated with 1x104 U mouse IFNalpha for 90 minutes.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40666", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40666/samples/"}