4ArrayExpress Uploaderarrayexpress_sidhumanJurkat1 uM PD-0332991 for 15hrsJurkat1 uM PD-0332991 for 15hrsJurkatDMSO for 15hrsJurkatDMSO for 15hrsDND411 uM PD-0332991 for 15hrsDND411 uM PD-0332991 for 15hrsDND41DMSO for 15hrsDND41DMSO for 15hrsCUTL11 uM PD-0332991 for 15hrsCUTL11 uM PD-0332991 for 15hrsCUTL1DMSO for 15hrsCUTL1DMSO for 15hrsCEM1 uM PD-0332991 for 15hrsCEM1 uM PD-0332991 for 15hrsCEMDMSO for 15hrsCEMDMSO for 15hrs26460http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40635Cyclin D3 is critical hematopoiesis and loss of cyclin D3 leads to resistance to transformation of bone marrow progenitors by Notch1-IC....23079656/profile/8773/arrayexpressuploader216bonebone marrowcellgenomeDec.12, 2014Falseexpression-data-from-vehicle-or-pd-0332991-treatedE-GEOD-40635_A-AFFY-44Expression data from vehicle or PD-0332991 treated human T-ALL linesJul.12, 2014Cyclin D3 is critical hematopoiesis and loss of cyclin D3 leads to resistance to transformation of bone marrow progenitors by Notch1-IC. We used a small molecule targeting cyclin D3:CDK4/6 activity to study whether its inhibition is an effective therapeutic strategy. We analyzed T-ALL lines in vitro with PD-0332991 or vehicle control to determine genes affected by the drug. CEM, Jurkat, DND41, and CUTL-1 cell lines were treated with 1 uM PD-0332991 or DMSO for 15 hours prior to RNA extraction and hybridization to Human Genome U133 Plus 2.0 Affymetrix arrays.E-GEOD-40635http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40635/samples/