{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 4567, "factors": [{"GSM996763": {"VARIATION": "expressing empty vector"}}, {"GSM996764": {"VARIATION": "expressing full-length SV40 LT"}}, {"GSM996765": {"VARIATION": "expressing SV40 LT fragment T6"}}, {"GSM996766": {"VARIATION": "expressing SV40 LT fragment T8"}}, {"GSM996767": {"VARIATION": "expressing SV40 LT fragment T16"}}, {"GSM996763": {"VARIATION": "expressing empty vector"}}, {"GSM996764": {"VARIATION": "expressing full-length SV40 LT"}}, {"GSM996765": {"VARIATION": "expressing SV40 LT fragment T6"}}, {"GSM996766": {"VARIATION": "expressing SV40 LT fragment T8"}}, {"GSM996767": {"VARIATION": "expressing SV40 LT fragment T16"}}, {"GSM996763": {"VARIATION": "expressing empty vector"}}, {"GSM996764": {"VARIATION": "expressing full-length SV40 LT"}}, {"GSM996765": {"VARIATION": "expressing SV40 LT fragment T6"}}, {"GSM996766": {"VARIATION": "expressing SV40 LT fragment T8"}}, {"GSM996767": {"VARIATION": "expressing SV40 LT fragment T16"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins...", "pubmed_id": 23093934, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 15, "tags": ["cell", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-40567_A-AFFY-44", "slug": "transcriptional-perturbations-caused-by-sv40-large", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40567", "geo_gds_id": "", "name": "Transcriptional perturbations caused by SV40 large T antigen and its fragments", "created": "Sep.19, 2014", "summary": "The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins for efficient viral replication. The SV40 large T antigen (LT) contains several discrete functional domains that contribute to the viral life cycle, including the DNA binding and helicase domains.  In addition, the LT the C-terminal region is required for lytic infection in certain restrictive cell types. To understand how LT affects the host cell to facilitate viral replication, we expressed full-length or functional domains of LT in cells and identified interacting cellular proteins and performed expression profiling.  LT perturbed the expression of p53 target genes and subsets of cell-cycle dependent genes regulated by the DREAM and the B-Myb-MuvB complexes. To examine transcriptome perturbations directly in human cells, we generated expression constructs of each LT fragment. Full length and truncated LT containing N-terminal HA and FLAG epitope tags were expressed from the pMSCV retroviral vector and introduced into U-2 OS cells. Total RNA was isolated from three biological replicate U-2 OS stable cell lines and gene expression was assayed on Affymetrix Human Gene U133 Plus 2.0 arrays.", "geo_gse_id": "E-GEOD-40567", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40567/samples/"}