ArrayExpress Uploader04567expressing empty vectorexpressing full-length SV40 LTexpressing SV40 LT fragment T6expressing SV40 LT fragment T8expressing SV40 LT fragment T16expressing empty vectorexpressing full-length SV40 LTexpressing SV40 LT fragment T6expressing SV40 LT fragment T8expressing SV40 LT fragment T16expressing empty vectorexpressing full-length SV40 LTexpressing SV40 LT fragment T6expressing SV40 LT fragment T8expressing SV40 LT fragment T16arrayexpress_sid4The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins...23093934/profile/8773/arrayexpressuploader115cellgenomeDec.12, 2014FalseE-GEOD-40567_A-AFFY-44transcriptional-perturbations-caused-by-sv40-largehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40567Transcriptional perturbations caused by SV40 large T antigen and its fragmentsSep.19, 2014The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins for efficient viral replication. The SV40 large T antigen (LT) contains several discrete functional domains that contribute to the viral life cycle, including the DNA binding and helicase domains. In addition, the LT the C-terminal region is required for lytic infection in certain restrictive cell types. To understand how LT affects the host cell to facilitate viral replication, we expressed full-length or functional domains of LT in cells and identified interacting cellular proteins and performed expression profiling. LT perturbed the expression of p53 target genes and subsets of cell-cycle dependent genes regulated by the DREAM and the B-Myb-MuvB complexes. To examine transcriptome perturbations directly in human cells, we generated expression constructs of each LT fragment. Full length and truncated LT containing N-terminal HA and FLAG epitope tags were expressed from the pMSCV retroviral vector and introduced into U-2 OS cells. Total RNA was isolated from three biological replicate U-2 OS stable cell lines and gene expression was assayed on Affymetrix Human Gene U133 Plus 2.0 arrays.E-GEOD-40567humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40567/samples/