ArrayExpress Uploader0mousevehiclevehiclevehicleCDK4/6 inhibitor PD 0332991CDK4/6 inhibitor PD 0332991CDK4/6 inhibitor PD 03329917001arrayexpress_sid6D-cyclins represent components of cell cycle machinery. To test the efficacy of targeting D-cyclins in cancer treatment, we engineered...E-GEOD-40513/profile/8773/arrayexpressuploader16breastbreast cancercancercellproteinDec.12, 2014Falsegene-expression-profile-of-mouse-breast-cancer-v72E-GEOD-40513_A-AFFY-45Gene expression profile of mouse breast cancer V720 cells treated with vehicle or PD 0332991Nov.12, 2014D-cyclins represent components of cell cycle machinery. To test the efficacy of targeting D-cyclins in cancer treatment, we engineered mouse strains which allow acute and global ablation of individual D-cyclins in a living animal. Ubiquitous shutdown of cyclin D1 or inhibition of cyclin D associated kinase activity in mice bearing ErbB2-driven mammary carcinomas halted cancer progression and triggered tumor-specific senescence, without compromising the animals' health. Ablation of cyclin D3 in mice bearing T-cell acute lymphoblastic leukemias (T-ALL) triggered tumorspecific apoptosis. Such selective killing of leukemic cells can be also achieved by inhibiting cyclin D associated kinase activity in mouse and human T-ALL models. Hence, contrary to what one might expect from ablation of a cell cycle protein, acute shutdown of a D-cyclin leads not only to cell cycle arrest, but it also triggers tumor cell senescence or apoptosis, and it affects different tumor types through distinct cellular mechanisms. Inhibiting cyclin D-activity represents a highly-selective anticancer strategy which specifically targets cancer cells without significantly affecting normal tissues. Mouse breast cancer V720 cells were cultured in the presence of the CDK4/6 inhibitor PD 0332991 (PD; 1 microM) or vehicle (VO) for 24 hrs. Experiment was done in biological triplicate. A total of 6 RNA samples (3 vehicle treated and 3 PD 0332991 treated samples) were used for microarray expression analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40513http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40513/samples/