ArrayExpress Uploader0humanvehicle vehicle vehicle PD 03329911PD 03329911PD 033299114566arrayexpress_sid4D-cyclins represent components of cell cycle machinery. To test the efficacy of targeting D-cyclins in cancer treatment, we engineered...E-GEOD-40512/profile/8773/arrayexpressuploader26cancercelllineproteinDec.12, 2014FalseE-GEOD-40512_A-AFFY-44transcription-profiling-by-array-of-kopt-k1-t-allTranscription profiling by array of KOPT-K1 T-ALL cells after treatment with PD 0332991Sep.19, 2014D-cyclins represent components of cell cycle machinery. To test the efficacy of targeting D-cyclins in cancer treatment, we engineered mouse strains which allow acute and global ablation of individual D-cyclins in a living animal. Ubiquitous shutdown of cyclin D1 or inhibition of cyclin D associated kinase activity in mice bearing ErbB2-driven mammary carcinomas halted cancer progression and triggered tumor-specific senescence, without compromising the animals' health. Ablation of cyclin D3 in mice bearing T-cell acute lymphoblastic leukemias (T-ALL) triggered tumorspecific apoptosis. Such selective killing of leukemic cells can be also achieved by inhibiting cyclin D associated kinase activity in mouse and human T-ALL models. Hence, contrary to what one might expect from ablation of a cell cycle protein, acute shutdown of a D-cyclin leads not only to cell cycle arrest, but it also triggers tumor cell senescence or apoptosis, and it affects different tumor types through distinct cellular mechanisms. Inhibiting cyclin D-activity represents a highly-selective anticancer strategy which specifically targets cancer cells without significantly affecting normal tissues. A human T-ALL cell line KOPTK1 cells were cultured in the presence of the CDK4/6 inhibitor PD 0332991 (PD; 1 microM) or vehicle (VO) for 48 hrs. Experiment was done in biological triplicate. A total of 6 RNA samples (3 vehicle treated and 3 PD 0332991 treated samples) were used for microarray expression analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40512http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40512/samples/