{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM994646": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "monosomal RNA"}}, {"GSM994646": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "monosomal RNA"}}, {"GSM994648": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "polysomal RNA"}}, {"GSM994648": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "polysomal RNA"}}, {"GSM994646": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "monosomal RNA"}}, {"GSM994646": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "monosomal RNA"}}, {"GSM994648": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "polysomal RNA"}}, {"GSM994648": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "polysomal RNA"}}, {"GSM994654": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "monosomal RNA"}}, {"GSM994654": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "monosomal RNA"}}, {"GSM994656": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "polysomal RNA"}}, {"GSM994656": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "polysomal RNA"}}, {"GSM994654": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "monosomal RNA"}}, {"GSM994654": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "monosomal RNA"}}, {"GSM994656": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "polysomal RNA"}}, {"GSM994656": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "polysomal RNA"}}, {"GSM994662": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "total RNA"}}, {"GSM994662": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "total RNA"}}, {"GSM994662": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "total RNA"}}, {"GSM994662": {"ANTIBODY": "not specified", "VARIATION": "wild type", "FRACTION": "total RNA"}}, {"GSM994666": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "total RNA"}}, {"GSM994666": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "total RNA"}}, {"GSM994666": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "total RNA"}}, {"GSM994666": {"ANTIBODY": "not specified", "VARIATION": "hnRNP E1 knockdown (E1KD)", "FRACTION": "total RNA"}}, {"GSM994670": {"ANTIBODY": "hnRNP E1", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994670": {"ANTIBODY": "hnRNP E1", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994670": {"ANTIBODY": "hnRNP E1", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994670": {"ANTIBODY": "hnRNP E1", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994674": {"ANTIBODY": "IgG", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994674": {"ANTIBODY": "IgG", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994674": {"ANTIBODY": "IgG", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}, {"GSM994674": {"ANTIBODY": "IgG", "VARIATION": "wild type", "FRACTION": "cytosolic RNA"}}], "id": 6998, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Regulation of gene expression at the post-transcriptional level plays an indispensable role during TGFbeta-induced EMT and metastasis....", "geo_gse_id": "E-GEOD-40466", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 32, "tags": ["genome", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-profiling-of-tgf-beta-induced-and-hnrnp", "geo_id_plat": "E-GEOD-40466_A-AFFY-45", "name": "Expression profiling of TGF-beta-induced and hnRNP E1-mediated epithelial-mesenchymal transition", "created": "Nov.12, 2014", "summary": "Regulation of gene expression at the post-transcriptional level plays an indispensable role during TGFbeta-induced EMT and metastasis. This regulation involves a transcript-selective translational regulatory pathway in which a ribonucleoprotein (mRNP) complex, consisting of heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) and eukaryotic elongation factor 1A1 (eEF1A1), binds to a 3\u2019-UTR regulatory BAT (TGF\u03b2 activated translation) element and silences translation of Dab2 and ILEI mRNAs, two transcripts which are involved in mediating EMT. TGFbeta activates a kinase cascade terminating in the phosphorylation of hnRNP E1, by isoform-specific stimulation of protein kinase B/Akt2, inducing the release of the mRNP complex from the 3\u2019-UTR element, resulting in the reversal of translational silencing and increased expression of Dab2 and ILEI transcripts. We adopted a combinatorial approach involving polysome profiling and RIP-Chip analyses using hnRNP E1 and filtered the array data based on the regulatory mechanism of Dab2 and ILEI. This led to the identification and validation of a cohort of target mRNAs that follow the same pattern of regulation as Dab2 and ILEI. To identify potential target mRNA transcripts that are translationally regulated by hnRNP E1 in a TGF-beta-dependent manner, we adopted a combinatorial approach involving expression profiling analyses and RNA immunoprecipitation analysis (RIP-Chip). We performed a screen using: 1) total mRNA and 2) RNA isolated from monosomal (non-translating) versus polysomal (translating) fractions from TGF-beta-treated (24 h) and non-treated NMuMG cells and from the hnRNP E1 knockdown derivative (E1KD), that undergo constitutive EMT even in the absence of TGF-beta. In addition, we screened for transcripts that selectively interact with hnRNP E1 in NMuMG cells under unstimulated conditions and subsequently lose their temporal association following TGF-beta stimulation. The samples were individually hybridized to Affymetrix GeneChip\u00ae Mouse Genome 430 2.0 arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40466", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40466/samples/"}