ArrayExpress Uploader0mousenot specifiedwild typemonosomal RNAnot specifiedwild typemonosomal RNAnot specifiedwild typepolysomal RNAnot specifiedwild typepolysomal RNAnot specifiedwild typemonosomal RNAnot specifiedwild typemonosomal RNAnot specifiedwild typepolysomal RNAnot specifiedwild typepolysomal RNAnot specifiedhnRNP E1 knockdown (E1KD)monosomal RNAnot specifiedhnRNP E1 knockdown (E1KD)monosomal RNAnot specifiedhnRNP E1 knockdown (E1KD)polysomal RNAnot specifiedhnRNP E1 knockdown (E1KD)polysomal RNAnot specifiedhnRNP E1 knockdown (E1KD)monosomal RNAnot specifiedhnRNP E1 knockdown (E1KD)monosomal RNAnot specifiedhnRNP E1 knockdown (E1KD)polysomal RNAnot specifiedhnRNP E1 knockdown (E1KD)polysomal RNAnot specifiedwild typetotal RNAnot specifiedwild typetotal RNAnot specifiedwild typetotal RNAnot specifiedwild typetotal RNAnot specifiedhnRNP E1 knockdown (E1KD)total RNAnot specifiedhnRNP E1 knockdown (E1KD)total RNAnot specifiedhnRNP E1 knockdown (E1KD)total RNAnot specifiedhnRNP E1 knockdown (E1KD)total RNAhnRNP E1wild typecytosolic RNAhnRNP E1wild typecytosolic RNAhnRNP E1wild typecytosolic RNAhnRNP E1wild typecytosolic RNAIgGwild typecytosolic RNAIgGwild typecytosolic RNAIgGwild typecytosolic RNAIgGwild typecytosolic RNA6998arrayexpress_sid6Regulation of gene expression at the post-transcriptional level plays an indispensable role during TGFbeta-induced EMT and metastasis....E-GEOD-40466/profile/8773/arrayexpressuploader332genomeproteinDec.12, 2014Falseexpression-profiling-of-tgf-beta-induced-and-hnrnpE-GEOD-40466_A-AFFY-45Expression profiling of TGF-beta-induced and hnRNP E1-mediated epithelial-mesenchymal transitionNov.12, 2014Regulation of gene expression at the post-transcriptional level plays an indispensable role during TGFbeta-induced EMT and metastasis. This regulation involves a transcript-selective translational regulatory pathway in which a ribonucleoprotein (mRNP) complex, consisting of heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) and eukaryotic elongation factor 1A1 (eEF1A1), binds to a 3’-UTR regulatory BAT (TGFβ activated translation) element and silences translation of Dab2 and ILEI mRNAs, two transcripts which are involved in mediating EMT. TGFbeta activates a kinase cascade terminating in the phosphorylation of hnRNP E1, by isoform-specific stimulation of protein kinase B/Akt2, inducing the release of the mRNP complex from the 3’-UTR element, resulting in the reversal of translational silencing and increased expression of Dab2 and ILEI transcripts. We adopted a combinatorial approach involving polysome profiling and RIP-Chip analyses using hnRNP E1 and filtered the array data based on the regulatory mechanism of Dab2 and ILEI. This led to the identification and validation of a cohort of target mRNAs that follow the same pattern of regulation as Dab2 and ILEI. To identify potential target mRNA transcripts that are translationally regulated by hnRNP E1 in a TGF-beta-dependent manner, we adopted a combinatorial approach involving expression profiling analyses and RNA immunoprecipitation analysis (RIP-Chip). We performed a screen using: 1) total mRNA and 2) RNA isolated from monosomal (non-translating) versus polysomal (translating) fractions from TGF-beta-treated (24 h) and non-treated NMuMG cells and from the hnRNP E1 knockdown derivative (E1KD), that undergo constitutive EMT even in the absence of TGF-beta. In addition, we screened for transcripts that selectively interact with hnRNP E1 in NMuMG cells under unstimulated conditions and subsequently lose their temporal association following TGF-beta stimulation. The samples were individually hybridized to Affymetrix GeneChip® Mouse Genome 430 2.0 arrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40466http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40466/samples/