ArrayExpress Uploader0mouse9.5-day embryoswild typeno serum9.5-day embryoswild typeserum for 30min9.5-day embryoswild typeserum for 90min9.5-day embryosMed23KOno serum9.5-day embryosMed23KOserum for 30min9.5-day embryosMed23KOserum for 90mincell line derived from embryonot specifiednonecell line derived from embryonot specifiedMAL overexpressioncell line derived from embryonot specifiedMED23 deficiencycell line derived from embryonot specifiedMAL overexpression and MED23 deficiency6985arrayexpress_sid6We examined how MED23 regulates SRF-targeted genes by comparing WT and Med23 KO mouse embryonic fibroblasts (MEFs) in the presence or...E-GEOD-40286/profile/8773/arrayexpressuploader310adipocytecellserumDec.12, 2014Falsethe-mediator-med23-plays-opposing-roles-in-directiE-GEOD-40286_A-AFFY-45The Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiationNov.12, 2014We examined how MED23 regulates SRF-targeted genes by comparing WT and Med23 KO mouse embryonic fibroblasts (MEFs) in the presence or absence of serum-stimulation (for 30 and 90 min). To investigate whether MAL antagonizes MED23 in the adipocyte lineage program, we compared the gene expression changes resulting from Mal overexpression (oxMal) and Med23 deficiency (siMed23) in 10T1/2 cell. In MEF cells, we examined SRF-dependent gene expression in three time points (0, 30, 90min) and two conditions (WT and KO). In 10T1/2 cells, we used three treatment conditions (Mal overexpression, Med23 knockdown, and treatment of both Mal overexpression and Med23 knockdown) with control (no treatment).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40286http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40286/samples/