Dataset: The Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation
We examined how MED23 regulates SRF-targeted genes by comparing WT and Med23 KO mouse embryonic fibroblasts (MEFs) in the presence or...
We examined how MED23 regulates SRF-targeted genes by comparing WT and Med23 KO mouse embryonic fibroblasts (MEFs) in the presence or absence of serum-stimulation (for 30 and 90 min). To investigate whether MAL antagonizes MED23 in the adipocyte lineage program, we compared the gene expression changes resulting from Mal overexpression (oxMal) and Med23 deficiency (siMed23) in 10T1/2 cell. In MEF cells, we examined SRF-dependent gene expression in three time points (0, 30, 90min) and two conditions (WT and KO). In 10T1/2 cells, we used three treatment conditions (Mal overexpression, Med23 knockdown, and treatment of both Mal overexpression and Med23 knockdown) with control (no treatment).
- Species:
- mouse
- Samples:
- 10
- Source:
- E-GEOD-40286
- Updated:
- Dec.12, 2014
- Registered:
- Nov.12, 2014
Sample | TREATMENT | VARIATION | DEVELOPMENTAL STAGE |
---|---|---|---|
GSM990680 | no serum | wild type | 9.5-day embryos |
GSM99068 | serum for 30min | wild type | 9.5-day embryos |
GSM990682 | serum for 90min | wild type | 9.5-day embryos |
GSM990683 | no serum | Med23KO | 9.5-day embryos |
GSM990684 | serum for 30min | Med23KO | 9.5-day embryos |
GSM990685 | serum for 90min | Med23KO | 9.5-day embryos |
GSM990686 | none | not specified | cell line derived from embryo |
GSM990687 | MAL overexpression | not specified | cell line derived from embryo |
GSM990688 | MED23 deficiency | not specified | cell line derived from embryo |
GSM990689 | MAL overexpression and MED23 deficiency | not specified | cell line derived from embryo |