{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM989635": {"IFN TYPE1": "IFN\u03b11-DC"}}, {"GSM989636": {"IFN TYPE1": "IFN\u03b12-DC"}}, {"GSM989637": {"IFN TYPE1": "IFN\u03b18-DC"}}, {"GSM989638": {"IFN TYPE1": "IFN\u03b121-DC"}}, {"GSM989639": {"IFN TYPE1": "IFN\u03b2-DC"}}], "id": 4556, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Type I interferon (IFN) is a family of 15 cytokines (in human 13\u03b1, 1\u03b2,1\u03c9) which exert several cellular functions through the binding to a...", "geo_gse_id": "E-GEOD-40268", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 5, "tags": ["cell", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-5-ifn-type-i-derived-dendriti", "geo_id_plat": "E-GEOD-40268_A-AFFY-44", "name": "Expression data from 5 IFN type I-derived Dendritic Cells", "created": "Sep.19, 2014", "summary": "Type I interferon (IFN) is a family of 15 cytokines (in human 13\u03b1, 1\u03b2,1\u03c9) which exert several cellular functions through the binding to a common receptor. Despite the initial activation of the same Jak/Stat signalling pathway, the cellular response may be different depending on the type I IFN subtype. We investigated the activity of different type I IFN subtypes - IFN\u03b11, \u03b12, \u03b18, \u03b121, \u03c9 and \u03b2- on the differentiation of DC. Transcriptome analyses identified two distinct groups, the IFN\u03b1/\u03c9-DC and the IFN\u03b2-DC. 78 genes, 7 chemokines and expression levels of cell surface markers characteristic of DC distinguished IFN\u03b1-DC and IFN\u03b2-DC. These differences are unlikely to impact the efficacy of T cell functional response since IFN\u03b12-DC and IFN\u03b2-DC were equipotent in inducing the proliferation and the polarization of allogenic na\u00efve CD4 T cells into Th1 cells and in stimulating autologous memory CD4 or CD8 T cells. In contrast, IFN\u03b12-DC were found to be more efficient than IFN\u03b2-DC in the phagocytic uptake of dead cells. Human blood monocytes were differentiated in DC by using 5 differents IFN type I (IFN\u03b12, \u03b11, \u03b18, \u03b121 and \u03b2). After 3 days of differentiation RNA were extracted and analyzed by affymetrix microarray.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40268", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40268/samples/"}