{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM989360": {"TREATMENT": "Scramble ASO"}}, {"GSM989360": {"TREATMENT": "Scramble ASO"}}, {"GSM989360": {"TREATMENT": "Scramble ASO"}}, {"GSM989360": {"TREATMENT": "Scramble ASO"}}, {"GSM989364": {"TREATMENT": "MiR-29a ASO"}}, {"GSM989364": {"TREATMENT": "MiR-29a ASO"}}, {"GSM989364": {"TREATMENT": "MiR-29a ASO"}}, {"GSM989364": {"TREATMENT": "MiR-29a ASO"}}], "id": 6982, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Adult BALB/c female mice were injected intraperitoneally with a single dose at 20 mg per kg of antisense oligonucleotide either against...", "geo_gse_id": "E-GEOD-40261", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["genome", "liver"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "hepatic-gene-expression-changes-following-antisens", "geo_id_plat": "E-GEOD-40261_A-AFFY-45", "name": "Hepatic gene expression changes following antisense oligonucleotide-based inhibition of miR-29a", "created": "Nov.12, 2014", "summary": "Adult BALB/c female mice were injected intraperitoneally with a single dose at 20 mg per kg of antisense oligonucleotide either against miR-29a (5\u2019-TAACCGATTTCAGATGGTGCTA-3\u2019) or against a scrambled sequence (5\u2019-TCATTGGCATGTACCATGCAGCT-3\u2019 Antisense oligonucleotides contained 2\u2019-O-methoxyethyl (2\u2019-MOE), 2\u2019-flouro (2\u2019-F) 2'-alpha-flouro units with a phosphorothioate backbone (Regulus Therapeutics). Six days following the injection, liver was isolated, total RNA was prepared as described above, and the RNA was amplified and biotinylated using the MessageAmp Premier kit (Ambion). Samples (n=4 each experimental and control) were hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays in the Children\u2019s Hospital of Philadelphia Nucleic Acids Core Facility\u00a0and analyzed with the assistance of the Penn Bioinformatics Core. Probe intensities were normalized using the GCRMA method and the significance of the log2-transformed, GCRMA-normalized signal intensities was determined using SAM Adult mice injected with anti-miR-29a or scrambled control ASO, n=4 per group", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40261", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40261/samples/"}