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<biogps><data><item key="owner">ArrayExpress Uploader</item><item key="pop_total">0</item><item key="species">mouse</item><item key="factors"><item><item key="GSM981274"><item key="CELL TYPE">B220+ CD23+ spleen B cells</item></item></item><item><item key="GSM981274"><item key="CELL TYPE">B220+ CD23+ spleen B cells</item></item></item><item><item key="GSM981274"><item key="CELL TYPE">B220+ CD23+ spleen B cells</item></item></item><item><item key="GSM981277"><item key="CELL TYPE">B220- CD138+ bone marrow plasma cells</item></item></item><item><item key="GSM981277"><item key="CELL TYPE">B220- CD138+ bone marrow plasma cells</item></item></item><item><item key="GSM981277"><item key="CELL TYPE">B220- CD138+ bone marrow plasma cells</item></item></item></item><item key="id">6972</item><item key="ownerprofile_id">arrayexpress_sid</item><item key="platform">6</item><item key="summary_wrapped">CD138+ B220- plasma cells were sorted from bone marrow and B220+ CD23+ mature follicular B cells were sorted from the spleens.  Plasma...</item><item key="geo_gse_id">E-GEOD-39916</item><item key="owner_profile">/profile/8773/arrayexpressuploader</item><item key="factor_count">1</item><item key="sample_count">6</item><item key="tags"><item>bone</item><item>bone marrow</item></item><item key="lastmodified">Dec.12, 2014</item><item key="is_default">False</item><item key="geo_gds_id"/><item key="slug">expression-data-from-murine-bone-marrow-resident-p</item><item key="geo_id_plat">E-GEOD-39916_A-AFFY-45</item><item key="name">Expression data from murine bone marrow-resident plasma cells and spleen mature follicular B cells</item><item key="created">Nov.12, 2014</item><item key="summary">CD138+ B220- plasma cells were sorted from bone marrow and B220+ CD23+ mature follicular B cells were sorted from the spleens.  Plasma cells were sorted from C57BL/6 mice 7 days after boosting with antigen, with mice first primed with an i.p. injection of KLH/IFA followed by boost at day 21 with KLH/PBS i.p. Mature B cells were sorted from antigen-na&#239;ve C57BL/6 mice. We compared expression profiles of plasma cells and mature B cells to identify differentially expressed transcripts. Seven days after boosting with KLH, plasma cells were enriched by CD138-PE/anti-PE magnetic bead enrichment and then sorted to purity.  Affymetrix microarrays were performed on 50,000-70,000 plasma cells and 2,000,000-3,000,000 na&#239;ve B cells.</item><item key="source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39916</item><item key="sample_source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39916/samples/</item></data></biogps>
