{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM980699": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "DMSO"}}, {"GSM980700": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "control-768"}}, {"GSM98070": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.5 uM"}}, {"GSM980702": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.25 uM"}}, {"GSM980703": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "DMSO"}}, {"GSM980704": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "control-768"}}, {"GSM980705": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.5 uM"}}, {"GSM980706": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.25 uM"}}, {"GSM980699": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "DMSO"}}, {"GSM980700": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "control-768"}}, {"GSM98070": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.5 uM"}}, {"GSM980702": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.25 uM"}}, {"GSM980703": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "DMSO"}}, {"GSM980704": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "control-768"}}, {"GSM980705": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.5 uM"}}, {"GSM980706": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.25 uM"}}, {"GSM980699": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "DMSO"}}, {"GSM980700": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "control-768"}}, {"GSM98070": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.5 uM"}}, {"GSM980702": {"STATE": "resting", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.25 uM"}}, {"GSM980703": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "DMSO"}}, {"GSM980704": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "control-768"}}, {"GSM980705": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.5 uM"}}, {"GSM980706": {"STATE": "activated", "GROWN IN THE PRESENCE OF": "I-BET-762 at 0.25 uM"}}], "id": 6970, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Bromodomain-containing proteins bind acetylated lysine residues on histone tails and are involved in  the recruitment of additional...", "geo_gse_id": "E-GEOD-39886", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 24, "tags": ["cell", "cytokine", "encephalomyelitis", "histone", "lysine", "macrophage", "multiple sclerosis"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "selective-inhibition-of-cd4-t-cell-cytokine-produc", "geo_id_plat": "E-GEOD-39886_A-AFFY-45", "name": "Selective inhibition of CD4+ T-cell cytokine production and autoimmunity by BET protein and c-Myc inhibitors", "created": "Nov.12, 2014", "summary": "Bromodomain-containing proteins bind acetylated lysine residues on histone tails and are involved in  the recruitment of additional factors that mediate histone modifications and enable transcription. A compound, I-BET-762, that inhibits binding of an acetylated histone peptide to BRD4 and other proteins of the BET (bromodomain and extra-terminal domain) family, was previously shown to suppress the production of pro-inflammatory proteins by macrophages and block acute inflammation in mice. Here we investigate the effect of I-BET-762 on T cell function. We show that treatment of na\u00efve CD4+ T cells with I-BET-762 during early differentiation modulates subsequent cytokine production, and inhibits the ability of Th1-skewed cells to induce autoimmune pathogenesis in a model  of experimental autoimmune encephalomyelitis (EAE) in vivo. The suppressive effects of I-BET-762 on T-cell mediated inflammation were not due to inhibition of expression of the pro-inflammatory cytokines, IFN-. or IL-17, but correlated with the ability to suppress GM-CSF production from CNS-infiltrating T cells, resulting in decreased recruitment of macrophages and granulocytes. The effects of I-BET-762 were distinct from those of the fumarate ester, dimethyl fumarate (DMF), a candidate drug for treatment of multiple sclerosis (MS). Our data suggest that I-BET and DMF could have complementary roles in the treatment of MS, and provide a strong rationale for inhibitors of BET-family proteins in the treatment of autoimmune diseases, based on their dual ability to suppress granulocyte and macrophage recruitment by T cells as well as production of pro-inflammatory proteins by macrophages. RNA from resting or activated CD4+ T cells grown in the presence of a control substance (DMSO or Control-768) or two different concentrations of I-BET-762, was hybridized to the chip.  There are 3 biological replicates for a total of 2 (cell states) x 4 (conditions) x 3 (replicates) = 24 samples.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39886", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39886/samples/"}