ArrayExpress Uploader0mouseWTWTWTGATA3 KOGATA3 KOGATA3 KO8637arrayexpress_sid8The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (Treg cells). To gain insights...22922362E-GEOD-39864/profile/8773/arrayexpressuploader16cellproteinDec.12, 2014FalseE-GEOD-39864_A-AFFY-36treg-specific-gata3-knock-out-arrayTreg specific Gata3 knock out arrayNov.24, 2014The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (Treg cells). To gain insights into the molecular mechanisms of Foxp3 mediated gene expression we purified Foxp3 complexes and explored their composition. Biochemical and mass-spectrometric analyses revealed that Foxp3 forms multi-protein complexes of 400-800 kDa or larger and identified 361 associated proteins ~30% of which are transcription-related. Foxp3 directly regulates expression of a large proportion of the genes encoding its co-factors. Reciprocally, some transcription factor partners of Foxp3 facilitate its expression. Functional analysis of Foxp3 cooperation with one such partner, Gata3, provided further evidence for a network of transcriptional regulation afforded by Foxp3 and its associates to control distinct aspects of Treg cell biology. Gene expression profile of Treg specific knock-out of Gata3 vs. their littermate controls were analyzed to gain insight into Gata3 dependendent genes in Treg cells. Treg cells (CD4+CD25+YFP+) sorted from Gata3F/FFoxp3YFP-Cre and Gata3F/+Foxp3YFP-Cre mice (three mice in each group) followed by microarray analyses.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39864http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39864/samples/