{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM980505": {"VARIATION": "WT"}}, {"GSM980505": {"VARIATION": "WT"}}, {"GSM980505": {"VARIATION": "WT"}}, {"GSM980508": {"VARIATION": "GATA3 KO"}}, {"GSM980508": {"VARIATION": "GATA3 KO"}}, {"GSM980508": {"VARIATION": "GATA3 KO"}}], "id": 8637, "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (Treg cells). To gain insights...", "pubmed_id": 22922362, "geo_gse_id": "E-GEOD-39864", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-39864_A-AFFY-36", "slug": "treg-specific-gata3-knock-out-array", "geo_gds_id": "", "name": "Treg specific Gata3 knock out array", "created": "Nov.24, 2014", "summary": "The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (Treg cells). To gain insights into the molecular mechanisms of Foxp3 mediated gene expression we purified Foxp3 complexes and explored their composition. Biochemical and mass-spectrometric analyses revealed that Foxp3 forms multi-protein complexes of 400-800 kDa or larger and identified 361 associated proteins ~30% of which are transcription-related. Foxp3 directly regulates expression of a large proportion of the genes encoding its co-factors.  Reciprocally, some transcription factor partners of Foxp3 facilitate its expression. Functional analysis of Foxp3 cooperation with one such partner, Gata3, provided further evidence for a network of transcriptional regulation afforded by Foxp3 and its associates to control distinct aspects of Treg cell biology. Gene expression profile of Treg specific knock-out of Gata3 vs. their littermate controls were analyzed to gain insight into Gata3 dependendent genes in Treg cells. Treg cells (CD4+CD25+YFP+) sorted from Gata3F/FFoxp3YFP-Cre and Gata3F/+Foxp3YFP-Cre mice (three mice in each group) followed by microarray analyses.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39864", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39864/samples/"}