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Home › Dataset Library › Expression data of cystic fibrosis and non-cystic fibrosis airway cell lines under oxidative stress

Dataset: Expression data of cystic fibrosis and non-cystic fibrosis airway cell lines under oxidative stress

CF's physiopathology is poorly explained by the mutation alone. The oxydative stress could be a major factor of this illness . Study its...

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CF's physiopathology is poorly explained by the mutation alone. The oxydative stress could be a major factor of this illness . Study its impact on transcriptome's CF cell line could be ameliorate our understanding of the evolution of cystic fibrosis. we used microarray technology to evaluate under oxydative stress, the transcriptional state of an epithelial lung cell issued from a human with cystic fibrosis and to identify a set of modulated genes associated to survival cell processes. the two cell lines are cultivated to Air-liquid Interface for RNA extraction and hybridization on Affymetrix microarrays. Each condition is triplicated. For the oxidative stress conditions, the two cell lines are treated on apical site by 15 µl of DMNQ (2,3-dimethoxy-1,4-naphtoquinone) ,concentrated at 15 µM, during 24 hours before RNA extraction.

Species:
human

Samples:
12

Source:
E-GEOD-39843

Updated:
Dec.12, 2014

Registered:
Sep.19, 2014


Factors: (via ArrayExpress)
Sample CELL LINE GENOTYPE TREATMENT
GSM980285 CuFi Cystic Fibrosis deltaF508 mutation control
GSM980285 CuFi Cystic Fibrosis deltaF508 mutation control
GSM980285 CuFi Cystic Fibrosis deltaF508 mutation control
GSM980288 CuFi Cystic Fibrosis deltaF508 mutation DMNQ
GSM980288 CuFi Cystic Fibrosis deltaF508 mutation DMNQ
GSM980288 CuFi Cystic Fibrosis deltaF508 mutation DMNQ
GSM98029 NuLi no-Cystic Fibrosis deltaF508 mutation control
GSM98029 NuLi no-Cystic Fibrosis deltaF508 mutation control
GSM98029 NuLi no-Cystic Fibrosis deltaF508 mutation control
GSM980294 NuLi no-Cystic Fibrosis deltaF508 mutation DMNQ
GSM980294 NuLi no-Cystic Fibrosis deltaF508 mutation DMNQ
GSM980294 NuLi no-Cystic Fibrosis deltaF508 mutation DMNQ

Tags

  • apical
  • cell
  • cystic fibrosis
  • line
  • liquid
  • lung

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