ArrayExpress Uploader0mouseB220+ cells sorted out from bone marrow after 4 weeksB220+ cells sorted out from bone marrow after 4 weeksB220+ cells sorted out from bone marrow after 4 weeksPreB cellPreB cellProB cellProB cellCLP Ly6D- CLP cellCLP Ly6D- CLP cellCLP Ly6D+ CLP cellCLP Ly6D+ CLP cellB220+ cells sorted out from bone marrow after 4 weeksB220+ cells sorted out from bone marrow after 4 weeksB220+ cells sorted out from bone marrow after 4 weeks6959arrayexpress_sid6we have investigated molecular and functional properties in early B-lineage cells from Pax-5 deficient animals crossed to a B-lineage...E-GEOD-39554/profile/8773/arrayexpressuploader114bonebone marrowcellliverDec.12, 2014Falseexpression-data-from-early-b-cell-progenitors-inclE-GEOD-39554_A-AFFY-45Expression data from early B cell progenitors including CLP,ProB and PreB of Pax5 knockout and wild type C57Bl6 miceNov.12, 2014we have investigated molecular and functional properties in early B-lineage cells from Pax-5 deficient animals crossed to a B-lineage restricted reporter mouse. Gene expression analysis of ex vivo isolated progenitor cells revealed that Pax-5 deficiency has a minor impact on Bcell specification.By comparison of gene expression patterns in ex vivo isolated Pax-5 and Ebf-1 deficient progenitors, it was possible to identify a set of B-cell restricted genes dependent of Ebf-1 but not Pax-5, supporting the idea that B-cell specification and commitment is controlled by distinct regulatory networks. wt λ5 reporter+ and Pax5ko λ5reporter+ fetal liver from d15 embryos isolated and transplanted into sublethaly irradiated mice. After 4 weeks B220+λ5+ cells were sorted out from the bone marrow of the mice and subjected to microarray analyseshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39554http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39554/samples/