ArrayExpress Uploader02674in the presence of DHTLNCaP prostate cancer cellsdepleted of EZH2in the presence of DHTLNCaP prostate cancer cellstransfected with control siRNAin the presence of DHTLNCaP prostate cancer cellsdepleted of EZH2in the presence of DHTLNCaP prostate cancer cellstransfected with control siRNAin the presence of DHTLNCaP prostate cancer cellsdepleted of EZH2in the presence of DHTLNCaP prostate cancer cellstransfected with control siRNAunder hormone depleted conditionsLNCaP-abl (abl) prostate cancer cellsdepleted of EZH2under hormone depleted conditionsLNCaP-abl (abl) prostate cancer cellstransfected with control siRNAunder hormone depleted conditionsLNCaP-abl (abl) prostate cancer cellsdepleted of EZH2under hormone depleted conditionsLNCaP-abl (abl) prostate cancer cellstransfected with control siRNAunder hormone depleted conditionsLNCaP-abl (abl) prostate cancer cellsdepleted of EZH2under hormone depleted conditionsLNCaP-abl (abl) prostate cancer cellstransfected with control siRNAarrayexpress_sid4EZH2 is frequently over-expressed in aggressive and metastatic solid tumors, including castration resistant prostate cancer (CRPC). We...23239736E-GEOD-39452/profile/8773/arrayexpressuploader312androgencancercelllineprostateprostate cancersolidDec.12, 2014Falseexpression-data-of-ezh2-dependent-genes-in-prostatE-GEOD-39452_A-AFFY-44Expression data of EZH2-dependent genes in prostate cancer cell linesJul.12, 2014EZH2 is frequently over-expressed in aggressive and metastatic solid tumors, including castration resistant prostate cancer (CRPC). We sought to determine EZH2-dependent gene expression programmes in prostate cancer progression, and found an intriguing functional switch of EZH2 from a repressor to an activator during CRPC development. We used microarrays to detail the global profiling of gene expression that are differentially regulated upon EZH2 depletion in two different prostate cancer cell lines. The androgen-dependent prostate cancer cell line LNCaP and the LNCaP-derived androgen-independent cell line LNCaP-abl (abl) were used for this study, as their transcription profiles strongly resemble that of clinical androgen-dependent and castration resistant prostate tumors, respectively. EZH2 was silenced by specific siRNAs in both cell lines, and total RNA was extracted and hybridized on Affymetrix microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39452humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39452/samples/