Dataset: Differentially activated CD8 T cells in liver and gut

[u'Na\xefve, liver- and gut-activated CD8 OT-I T cells show differential migration behaviour. To analyze which genes could be responsible for different migration patterns, na\xefve, liver-activated and gut-activated CD8 T cells were isolated and compared for their gene expression profile. Gene expression profiles of na\xefve CD8 OT-I T cells versus liver-activated T cells and gut-activated T cells, respectively. Na\xefve CD8 OT-I T cells were isolated from lymph nodes and spleens of OT-I mice. CD8 OT-I T cells activated by liver-derived and gut-derived antigen for three days in vivo, positive for CFSE were sorted from livers of TF-OVA mice or from mesenteric lymph nodes of iFABP-OVA mice. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 \xb5g cRNA hybridized in duplicates for each of the three groups to the GeneChip arrays. Group1: na\xefve, Group2: liver-activated Group3: gut-activated. Lists of differentially regulated genes were created using High Performance Chip Data Analysis (HPCDA) with Bioretis database (', {u'a': {u'href': u'http://www.bioretis-analysis.de', u'target': u'_blank', u'$': u'http://www.bioretis-analysis.de'}}, u'). Worldwide data sharing is possible via Bioretis, please ask the authors.']

Species:

mouse

Samples:

6

Source:

E-GEOD-39449

Updated:

Dec.12, 2014

Registered:

Nov.12, 2014