{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM967943": {"CELL-TREATMENT": "none", "CELL TYPE": "ES cells", "TIME": "Day0"}}, {"GSM967943": {"CELL-TREATMENT": "none", "CELL TYPE": "ES cells", "TIME": "Day0"}}, {"GSM967943": {"CELL-TREATMENT": "none", "CELL TYPE": "ES cells", "TIME": "Day0"}}, {"GSM967946": {"CELL-TREATMENT": "+RA +Hh", "CELL TYPE": "motor neurons", "TIME": "Day5"}}, {"GSM967946": {"CELL-TREATMENT": "+RA +Hh", "CELL TYPE": "motor neurons", "TIME": "Day5"}}, {"GSM967946": {"CELL-TREATMENT": "+RA +Hh", "CELL TYPE": "motor neurons", "TIME": "Day5"}}, {"GSM967949": {"CELL-TREATMENT": "+RA +Hh +Dox +FGF", "CELL TYPE": "motor neurons caudalized with iCdx2 and FGF", "TIME": "Day5"}}, {"GSM967949": {"CELL-TREATMENT": "+RA +Hh +Dox +FGF", "CELL TYPE": "motor neurons caudalized with iCdx2 and FGF", "TIME": "Day5"}}, {"GSM967949": {"CELL-TREATMENT": "+RA +Hh +Dox +FGF", "CELL TYPE": "motor neurons caudalized with iCdx2 and FGF", "TIME": "Day5"}}], "id": 6953, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "We aim to understand the role that Cdx2 plays in specifying the rostro-caudal identity of differentiating motor neurons. We find that...", "geo_gse_id": "E-GEOD-39422", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 9, "tags": ["caudal", "chromatin", "motor neuron", "neuron"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "induced-cdx2-binding-in-progenitor-motor-neurons-a", "geo_id_plat": "E-GEOD-39422_A-AFFY-45", "name": "Induced Cdx2 binding in progenitor motor neurons and its effect on H3K27me3 chromatin domains [Affymetrix]", "created": "Nov.12, 2014", "summary": "We aim to understand the role that Cdx2 plays in specifying the rostro-caudal identity of differentiating motor neurons. We find that expressing Cdx2 in combination with FGF signaling is sufficient to produce motor neurons with a more caudal identity. ChIP-seq analysis of Cdx2 finds that it binds extensively throughout the Hox regions in progenitor motor neurons. Analysis of polycomb-associated chromatin over Hox regions in the subsequently generated motor neurons finds that Cdx2 binding corresponds to chromatin domains encompassing de-repressed caudal Hox genes. These results suggest a direct role for Cdx2 in specifying caudal motor neuron identity. Expression studies: Affymetrix arrays are used to profile gene expression in ES cells, RA/Hh-derived Day 5 motor neurons, and RA/Hh-derived motor neurons that have also been exposed to Dox (to activate iCdx2) and FGF.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39422", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39422/samples/"}