{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 2580, "factors": [{"GSM967338": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient VIII", "CELL TYPE": "leukocytes"}}, {"GSM967337": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient VIII", "CELL TYPE": "epithelial cells"}}, {"GSM967336": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient VIII", "CELL TYPE": "stromal cells"}}, {"GSM967335": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient VII", "CELL TYPE": "stromal cells"}}, {"GSM967334": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient VII", "CELL TYPE": "epithelial cells"}}, {"GSM967333": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient VII", "CELL TYPE": "leukocytes"}}, {"GSM967332": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient VI", "CELL TYPE": "stromal cells"}}, {"GSM96733": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient VI", "CELL TYPE": "leukocytes"}}, {"GSM967330": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient VI", "CELL TYPE": "epithelial cells"}}, {"GSM967329": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient V", "CELL TYPE": "leukocytes"}}, {"GSM967328": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient V", "CELL TYPE": "epithelial cells"}}, {"GSM967327": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient V", "CELL TYPE": "stromal cells"}}, {"GSM967326": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient IV", "CELL TYPE": "leukocytes"}}, {"GSM967325": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient IV", "CELL TYPE": "epithelial cells"}}, {"GSM967324": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient IV", "CELL TYPE": "stromal cells"}}, {"GSM967323": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient III", "CELL TYPE": "stromal cells"}}, {"GSM967322": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient III", "CELL TYPE": "leukocytes"}}, {"GSM96732": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient III", "CELL TYPE": "epithelial cells"}}, {"GSM967320": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient II", "CELL TYPE": "stromal cells"}}, {"GSM967319": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient II", "CELL TYPE": "leukocytes"}}, {"GSM967318": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient II", "CELL TYPE": "epithelial cells"}}, {"GSM967317": {"MARKER": "Epcam(-),CD45(-)", "INDIVIDUAL": "patient I", "CELL TYPE": "stromal cells"}}, {"GSM967316": {"MARKER": "Epcam(-),CD45(+)", "INDIVIDUAL": "patient I", "CELL TYPE": "leukocytes"}}, {"GSM967315": {"MARKER": "Epcam(+),CD45(-)", "INDIVIDUAL": "patient I", "CELL TYPE": "epithelial cells"}}], "pop_total": 0, "platform": 4, "summary_wrapped": "The survival of isolated metastatic cells and expansion into macroscopic tumour has been recognized as a limiting step for metastasis...", "pubmed_id": 23153532, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 24, "tags": ["cancer", "cell", "colorectal cancer", "disease"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-39395_A-AFFY-44", "slug": "expression-profiles-of-cell-populations-purified-f", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39395", "geo_gds_id": "", "name": "Expression profiles of cell populations purified from human CRC (3 ways)", "created": "Jul.12, 2014", "summary": "The survival of isolated metastatic cells and expansion into macroscopic tumour has been recognized as a limiting step for metastasis formation in several cancer types yet the determinants of this process remain largely uncharacterized. In colorectal cancer (CRC), we identify a transcriptional programme in tumour-associated stromal cells, which is intimately linked to a high risk of developing recurrent disease after therapy. A large proportion of CRCs display mutational inactivation of the TGF-beta pathway but paradoxically they are characterized by high TGF-beta production. In these tumours, TGF-beta instructs a transcriptional programme in stromal cells, which confers a high risk of developing metastatic disease. We purified by FACS [CD45(+),Epcam(-)], [CD45(-) Epcam(+)] and [CD45(-) Epcam(-)]  cell populations from fresh CRC samples and assessed their gene expression profiles Freshly obtained tumors from CRC patients (n=8) treated at Hospital del Mar (Barcelona, Spain) were minced and incubated with 0.1% Hyaluronidase and 0.1% Collagenase 1A. Pieces were then homogenized. Enzymatic reaction was stopped by adding 10% FBS and single cells were collected by sequential filtering. Cells were resuspended in Ammonium Chloride (0.15M) to lyse erythrocytes. Cells were stained with anti-hEpcam/TROP1-APC and anti-CD45-PE conjugated antibody. Dead cells were labeled with Propidium Iodide. Fluorescence Activated Cell Sorting (FACS) was used to separate cells.", "geo_gse_id": "E-GEOD-39395", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39395/samples/"}