{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2581, "factors": [{"GSM967314": {"PAIR": "pair 2", "TREATMENT": "TGB-beta1 for 8h"}}, {"GSM967313": {"PAIR": "pair 1", "TREATMENT": "TGB-beta1 for 8h"}}, {"GSM967312": {"PAIR": "pair 2", "TREATMENT": "non-treated"}}, {"GSM9673": {"PAIR": "pair 1", "TREATMENT": "non-treated"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The survival of isolated metastatic cells and expansion into macroscopic tumour has been recognized as a limiting step for metastasis...", "pubmed_id": 23153532, "geo_gse_id": "E-GEOD-39394", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cancer", "colon", "colorectal cancer", "disease", "mucosa"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-39394_A-AFFY-44", "slug": "expression-data-from-fibroblasts-treated-with-tgf", "geo_gds_id": "", "name": "Expression data from fibroblasts treated with TGF-Beta", "created": "Jul.12, 2014", "summary": "The survival of isolated metastatic cells and expansion into macroscopic tumour has been recognized as a limiting step for metastasis formation in several cancer types yet the determinants of this process remain largely uncharacterized. In colorectal cancer (CRC), we identify a transcriptional programme in tumour-associated stromal cells, which is intimately linked to a high risk of developing recurrent disease after therapy. A large proportion of CRCs display mutational inactivation of the TGF-beta pathway but paradoxically they are characterized by high TGF-beta production. In these tumours, TGF-beta instructs a transcriptional programme in stromal cells, which confers a high risk of developing metastatic disease. We quantified the association of TGF-beta-activated fibroblasts with disease progression. To this end, we used as surrogates the gene expression programme upregulated by addition of TGF-beta to normal colon mucosa-derived fibroblasts (CCD-Co-18) in culture. CCD-Co-18 were seeded at 60% confluence and treated with TGF-\u03b21. Gene expression profiles were measured in duplicate using HG-U133 plus 2.0. We used RMA background correction, quantile normalization and RMA summarization (Gautier et al., 2004). A TGF-\u03b2 response signature was obtained by selecting genes with limma P-value < 0.05 and at least two fold up-regulation in TGF-\u03b2 treated fibroblasts.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39394", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39394/samples/"}