ArrayExpress Uploader0mouseob/obovernight fasted, Diet 5058wild typeovernight fasted, Diet 5058ob/obad libitum fed, Diet 5058wild typead libitum fed, Diet 5058ob/obad libitum fed, Diet 5058wild typead libitum fed, Diet 5058ob/obad libitum fed, Diet 5058wild typead libitum fed, Diet 5058ob/obovernight fasted, Diet 5058wild typeovernight fasted, Diet 50586947arrayexpress_sid6Obesity-associated metabolic complications are generally considered to emerge from abnormalities in carbohydrate and lipid metabolism,...E-GEOD-39375/profile/8773/arrayexpressuploader210carbohydrateendoplasmic reticulumlipidliverobesityproteinDec.12, 2014Falsepolysome-profiling-in-liver-identifies-dynamic-regE-GEOD-39375_A-AFFY-45Polysome profiling in liver identifies dynamic regulation of endoplasmic reticulum translatome by obesity and fastingNov.12, 2014Obesity-associated metabolic complications are generally considered to emerge from abnormalities in carbohydrate and lipid metabolism, whereas the status of protein metabolism is not well studied. Here, we performed comparative polysome and associated transcriptional profiling analyses to study the dynamics and functional implications of endoplasmic reticulum (ER)-associated protein synthesis in the mouse liver under conditions of obesity and nutrient deprivation. lean wild type and genetically obese (ob/ob) mice with or without overnight fasting were sacrificed with their liver tissues removed. ER-associated polysomes were purified and analyzed by microarray.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39375http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39375/samples/