ArrayExpress Uploader01455endothelial cellinterferon gammafibroblastinterferon alphaendothelial cellcontrolendothelial cellinterferon alphafibroblastcontrolendothelial cellinterferon alphaendothelial cellinterferon alphaendothelial cellinterferon alphaendothelial cellcontrolfibroblastcontrolfibroblastinterferon gammaendothelial cellinterferon gammaendothelial cellcontrolendothelial cellinterferon betaendothelial cellcontrolfibroblastcontrolfibroblastinterferon gammaendothelial cellinterferon alphaendothelial cellinterferon gammafibroblastinterferon alphaendothelial cellinterferon betafibroblastinterferon alphaendothelial cellcontrolarrayexpress_sid3IFNs are highly pleiotropic cytokines also endowed with marked anti-angiogenic activity. In this study, the mRNA expression profiles of...17202376E-GEOD-3920/profile/8773/arrayexpressuploader223cellchemokineproteinDec.12, 2014Falsetranscription-profiling-of-human-endothelial-and-fE-GEOD-3920_A-AFFY-33Transcription profiling of human endothelial and fibroblast cells isolated from umbilical cord veins treated with interferons to identify genes regulated by interferonsJun.19, 2014IFNs are highly pleiotropic cytokines also endowed with marked anti-angiogenic activity. In this study, the mRNA expression profiles of endothelial cells (EC) exposed in vitro to IFN-alpha, IFN-beta, or; IFN-gamma were determined. We found that in HUVEC as well as in other EC types 175 genes were upregulated (>2-fold increase) by IFNs, including genes involved in the host response to RNA viruses, inflammation, and apoptosis. Interestingly, 41 genes showed a >5-fold higher induction by IFN-alpha in EC compared to human fibroblasts; among them, the gene encoding the angiostatic chemokine CXCL11 was selectively induced by IFN-alpha in EC along with other genes associated; with angiogenesis regulation, including CXCL10, TRAIL, and guanylate binding protein 1 (GBP-1). These transcriptional changes were confirmed and extended by quantitative PCR analysis and ELISA; whereas IFN-alpha and IFN-beta exerted virtually identical effects on transcriptome modulation, a differential gene regulation by type I and type II IFN emerged, especially as far as quantitative aspects were concerned. In vivo, IFN-alpha-producing tumors over-expressed murine CXCL10-11,; GBP-1 and TRAIL, with evidence of CXCL11 production by tumor-associated EC. Overall, these findings improve our understanding of the anti-angiogenic effects of IFNs by showing that these cytokines trigger an anti-angiogenic transcriptional program in EC. Moreover, we suggest that quantitative differences in the magnitude of the transcriptional activation of IFNresponsive genes could form the basis for cell-specific transcriptional signatures. In press, J. Immunol. 2007. Experiment Overall Design: comparison of interferon effects on endothelial cells (HUVEC, HMVEC) and fibrobloastshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3920humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3920/samples/