ArrayExpress Uploader0humannot specifiednone0 hheat stress1 hheat stress3 hheat stress4528arrayexpress_sid4Hyperthermia is widely used to treat patients with various cancers. Here, the effects of heat stress at 41°C for 30 min (mild...E-GEOD-39178/profile/8773/arrayexpressuploader24cellfibroblastgenomehandDec.12, 2014Falseidentification-of-genes-responsive-to-mild-hyper-4E-GEOD-39178_A-AFFY-44Identification of genes responsive to mild hyperthermia in human normal fibroblast OUMS-36 cellsSep.19, 2014Hyperthermia is widely used to treat patients with various cancers. Here, the effects of heat stress at 41°C for 30 min (mild hyperthermia) on the gene expression in OUMS-36 human normal fibroblast cells were investigated using an Affymetrix GeneChip system. The cells were treated with mild hyperthermia, followed by incubation for 0, 1, or 3 h at 37°C. No cell death was observed in the mild hyperthermia-treated cells. On the other hand, many genes that were differentially expressed by a factor 1.5 or greater were identified in the cells treated with the mild hyperthermia. OUMS-36 human normal fibroblast cells were treated with heat stress (41°C for 30 min), followed by incubation for 0, 1, or 3 h at 37°C. Non-treated cells served as the control. Total RNA samples were prepared from the cells, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was monitored by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39178http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39178/samples/