{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM957602": {"TIME AFTER TREATMENT": "not specified", "TREATMENT": "none"}}, {"GSM957603": {"TIME AFTER TREATMENT": "0 h", "TREATMENT": "heat stress"}}, {"GSM957604": {"TIME AFTER TREATMENT": "1 h", "TREATMENT": "heat stress"}}, {"GSM957605": {"TIME AFTER TREATMENT": "3 h", "TREATMENT": "heat stress"}}], "id": 4528, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Hyperthermia is widely used to treat patients with various cancers. Here, the effects of heat stress at 41\u00b0C for 30 min (mild...", "geo_gse_id": "E-GEOD-39178", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cell", "fibroblast", "genome", "hand"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "identification-of-genes-responsive-to-mild-hyper-4", "geo_id_plat": "E-GEOD-39178_A-AFFY-44", "name": "Identification of genes responsive to mild hyperthermia in human normal fibroblast OUMS-36 cells", "created": "Sep.19, 2014", "summary": "Hyperthermia is widely used to treat patients with various cancers. Here, the effects of heat stress at 41\u00b0C for 30 min (mild hyperthermia) on the gene expression in OUMS-36 human normal fibroblast cells were investigated using an Affymetrix GeneChip system. The cells were treated with mild hyperthermia, followed by incubation for 0, 1, or 3 h at 37\u00b0C. No cell death was observed in the mild hyperthermia-treated cells. On the other hand, many genes that were differentially expressed by a factor 1.5 or greater were identified in the cells treated with the mild hyperthermia. OUMS-36 human normal fibroblast cells were treated with heat stress (41\u00b0C for 30 min), followed by incubation for 0, 1, or 3 h at 37\u00b0C. Non-treated cells served as the control. Total RNA samples were prepared from the cells, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was monitored by an Affymetrix GeneChip\u00ae system with a Human Genome U133-plus 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39178", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39178/samples/"}