{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM954975 1": {}}, {"GSM954976 1": {}}], "id": 6940, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39064", "summary_wrapped": "Roles of mesothelial cells (MCs) are poorly understood during liver development and injury. We identified podoplanin (Pdpn) as a cell...", "pubmed_id": 23345421, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 2, "tags": ["cell", "genome", "liver", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-developing-mouse-livers", "geo_id_plat": "E-GEOD-39064_A-AFFY-45", "name": "Expression data from developing mouse livers", "created": "Nov.12, 2014", "summary": "Roles of mesothelial cells (MCs) are poorly understood during liver development and injury. We identified podoplanin (Pdpn) as a cell surface markers for mesothelial cells in E12.5 mouse developing liver. To identify genes uniquely expressed in MCs, we isolated MCs from E12.5 mouse livers by FACS using anti-Pdpn antibodies and performed microarray analysis. The E12.5 liver was digested with trypsin-EDTA and incubated with antibodies against Pdpn.  MCs were isolated as Pdpn+ population by FACS. Total RNA was extracted with RNAqueous Micro (Ambion) and the probes for the microarray were synthesized using the Ovation RNA amplification system V2 and FL-Ovation cDNA Biotin module V2 (Nugen). The labeled probes were hybridized with GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) and signals were analyzed with Genomic Suite software (Partek).", "geo_gse_id": "E-GEOD-39064", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39064/samples/"}