{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM950533": {"COMPOUND": "control"}}, {"GSM950533": {"COMPOUND": "control"}}, {"GSM950533": {"COMPOUND": "control"}}, {"GSM950536": {"COMPOUND": "EHTy"}}, {"GSM950536": {"COMPOUND": "EHTy"}}, {"GSM950536": {"COMPOUND": "EHTy"}}, {"GSM950539": {"COMPOUND": "HTy"}}, {"GSM950539": {"COMPOUND": "HTy"}}, {"GSM950539": {"COMPOUND": "HTy"}}], "id": 4513, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The anticarcinogenic activity of hydroxytyrosyl ethyl ether (HTy-Et) compared to its precursor hydroxytyrosol (HTy) has been studied in...", "geo_gse_id": "E-GEOD-38833", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["adenocarcinoma", "cancer", "cell", "colon", "colon adenocarcinoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-colon-caco-2-ce-2", "geo_id_plat": "E-GEOD-38833_A-AFFY-44", "name": "Transcription profiling of human colon Caco-2 cells treated with hydroxytyrosol (HTy) and hydroxytyrosyl ethyl ether (HTy-Et)", "created": "Sep.19, 2014", "summary": "The anticarcinogenic activity of hydroxytyrosyl ethyl ether (HTy-Et) compared to its precursor hydroxytyrosol (HTy) has been studied in human Caco-2 colon adenocarcinoma cells. Changes in global gene expression in Caco-2 cells exposed to HTy and HTy-Et for 24 h were evaluated using Affymetrix array. Microarray analysis showed that after exposure to HTy and HTy-Et, 451 and 977 genes respectively were differentially expressed compared with untreated cells (P < 0.005; FDR=0). Results show that HTy and its lipophilic ether derivative alter genes related with cancer prevention, which includes inducing cell cycle arrest, promoting apoptosis and enhancing xenobiotic metabolism. Caco-2 cells were treated with 10 \u03bcM of HTy and HTy-Et for 24 h, and total RNA from 3 biological replicates of each treatment together with the control cells, were isolated using a QIAGEN\u00ae RNeasy Mini Kit according to the manufacturer\u2019s instructions (Qiagen). RNA samples of each of the replicates were processed using Affymetrix Human HG-U133 Plus 2.0 microarrays (Affymetrix).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38833", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38833/samples/"}