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Home › Dataset Library › Cancer-Associated Fibroblasts Support Lung Cancer Stemness through Paracrine IGF-II/IGF1R/Nanog Signaling

Dataset: Cancer-Associated Fibroblasts Support Lung Cancer Stemness through Paracrine IGF-II/IGF1R/Nanog Signaling

The CLS1/CAF co-culture maintained the cancer stemness. This cancer stemness was lost when the CAF feeder cells were removed during...

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The CLS1/CAF co-culture maintained the cancer stemness. This cancer stemness was lost when the CAF feeder cells were removed during passaging. To investigate the specific signaling pathways and markers of lung CSCs, we analyzed the gene expression profile of the CLS1 sphere after CLS1/CAF co-culture and compared this profile with that obtained for CLS1 cells cultured without feeder cells through different passages (CLS1 p3, p6, and p14) and CLF1 CLS1 sphere generated by co-culture with CAF and remove CAF to mimic cancer cell differentiation. Four time points of the CLS1 after co-culture with CAF have been collected for microarray analysis:CLS1-2 p.3, p.6, p.8, p.14. We tried to compare theCLS1 sphere-regulated gene expression profiles with the CLS1-2 p.3, p.6, p.8, p.14 and CLF1 to identify the possible cross talk mechanisms of CLF1 and CLS1 and the signaling pathways that maintain stem cell properties.

Species:
human

Samples:
12

Source:
E-GEOD-38678

Updated:
Dec.12, 2014

Registered:
Jul.11, 2014


Factors: (via ArrayExpress)
Sample CULTURE CONDITION CELL LINE
GSM947459 not specified CLF1 cells
GSM947459 not specified CLF1 cells
GSM947459 not specified CLF1 cells
GSM947456 cultured without feeder cells through 14 passages CLS1 cells
GSM947455 cultured without feeder cells through 8 passages CLS1 cells
GSM947454 cultured without feeder cells through 6 passages CLS1 cells
GSM947454 cultured without feeder cells through 6 passages CLS1 cells
GSM947454 cultured without feeder cells through 6 passages CLS1 cells
GSM94745 cultured without feeder cells through 3 passages CLS1 cells
GSM947450 co-culture with CAF CLS1 sphere
GSM947450 co-culture with CAF CLS1 sphere
GSM947450 co-culture with CAF CLS1 sphere

Tags

  • cancer
  • cell
  • lung
  • stem cell

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