ArrayExpress Uploader0ratblastCD4+ myelin specific T cells2blastCD4+ myelin specific T cells2blastCD4+ myelin specific T cells2blastCD4+ myelin specific T cells2blastCD4+ myelin specific T cells2restingCD4+ myelin specific T cells7restingCD4+ myelin specific T cells7restingCD4+ myelin specific T cells7restingCD4+ myelin specific T cells7not specifiedsorted T cell from spleennot specifiednot specifiedsorted T cell from spleennot specified9570arrayexpress_sid13CNS autoimmunity is induced by autoreactive T cells reactive against CNS antigen. However how these T cells become able to transgress...22914092/profile/8773/arrayexpressuploader311braincellencephalomyelitisgenomemultiple sclerosismyelinmyelin basic proteinproteinspleenJun.26, 2015Falsegene-expression-profiling-of-cd4myelin-basic-protehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38645E-GEOD-38645_A-AFFY-25Gene expression profiling of CD4+myelin basic protein specific T cellsJan.12, 2015CNS autoimmunity is induced by autoreactive T cells reactive against CNS antigen. However how these T cells become able to transgress the blood brain barrier is not CNS autoimmunity is induced by autoreactive T cells reactive against CNS antigen. Here a gene expression profile of the pathogenic T cells in different functional states was performed. These studies were performed in a classical model of multiple sclerosis, experimental autoimmune encephalomyelitis in Lewis rats induced by transfer of CD4+myelin basic protein specific T cells. We found that on their way to the CNS T cells fundamentally reprogram their gene expression profile, by down-regulating their activation program and up-regulating cell locomotion molecules. Total RNA extracted from ex vitro myelin specific T cells (blasts and resting state, day 2 and 7 after antigen challenge respectively) or isolated from the spleen (3 days p.t.) was used to perform a genome-wide transcriptional profiling assay (Rat Genome 230, Affymetrix)-E-GEOD-38645http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38645/samples/