{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "rat", "factors": [{"GSM946766": {"STATE": "blast", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "2"}}, {"GSM946766": {"STATE": "blast", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "2"}}, {"GSM946766": {"STATE": "blast", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "2"}}, {"GSM946766": {"STATE": "blast", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "2"}}, {"GSM946766": {"STATE": "blast", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "2"}}, {"GSM946776": {"STATE": "resting", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "7"}}, {"GSM946776": {"STATE": "resting", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "7"}}, {"GSM946776": {"STATE": "resting", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "7"}}, {"GSM946776": {"STATE": "resting", "CELL TYPE": "CD4+ myelin specific T cells", "DAYS AFTER ANTIGEN CHALLENGE": "7"}}, {"GSM946784": {"STATE": "not specified", "CELL TYPE": "sorted T cell from spleen", "DAYS AFTER ANTIGEN CHALLENGE": "not specified"}}, {"GSM946784": {"STATE": "not specified", "CELL TYPE": "sorted T cell from spleen", "DAYS AFTER ANTIGEN CHALLENGE": "not specified"}}], "id": 9570, "ownerprofile_id": "arrayexpress_sid", "platform": 13, "summary_wrapped": "CNS autoimmunity is induced by autoreactive  T cells reactive against CNS antigen. However how these T cells become able to transgress...", "pubmed_id": 22914092, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 11, "tags": ["brain", "cell", "encephalomyelitis", "genome", "multiple sclerosis", "myelin", "myelin basic protein", "protein", "spleen"], "lastmodified": "Jun.26, 2015", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-profiling-of-cd4myelin-basic-prote", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38645", "geo_id_plat": "E-GEOD-38645_A-AFFY-25", "name": "Gene expression profiling of CD4+myelin basic protein specific T cells", "created": "Jan.12, 2015", "summary": "CNS autoimmunity is induced by autoreactive  T cells reactive against CNS antigen. However how these T cells become able to transgress the blood brain barrier is not CNS autoimmunity is induced by autoreactive  T cells reactive against CNS antigen. Here a gene expression profile of the pathogenic T cells in different functional states was performed. These studies were performed in a classical model of multiple sclerosis, experimental autoimmune encephalomyelitis in Lewis rats induced by transfer of CD4+myelin basic protein specific T cells. We found that on their way to the CNS T cells fundamentally reprogram their gene expression profile, by down-regulating their activation program and up-regulating cell locomotion molecules. Total RNA extracted from ex vitro myelin specific T cells (blasts and resting state, day 2 and 7 after antigen challenge respectively) or isolated from the spleen (3 days p.t.) was used to perform a genome-wide transcriptional profiling assay (Rat Genome 230, Affymetrix)-", "geo_gse_id": "E-GEOD-38645", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38645/samples/"}