{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM941679": {"TRANSFECTION": "Ala+TIMP-2"}}, {"GSM941678": {"TRANSFECTION": "TIMP-2"}}, {"GSM941677": {"TRANSFECTION": "EV control"}}, {"GSM941679": {"TRANSFECTION": "Ala+TIMP-2"}}, {"GSM941679": {"TRANSFECTION": "Ala+TIMP-2"}}, {"GSM941679": {"TRANSFECTION": "Ala+TIMP-2"}}, {"GSM941678": {"TRANSFECTION": "TIMP-2"}}, {"GSM941678": {"TRANSFECTION": "TIMP-2"}}, {"GSM941678": {"TRANSFECTION": "TIMP-2"}}, {"GSM941677": {"TRANSFECTION": "EV control"}}, {"GSM941677": {"TRANSFECTION": "EV control"}}, {"GSM941677": {"TRANSFECTION": "EV control"}}], "id": 2566, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "TIMP-2 is an endogenous angiogenesis inhibitor, i.e. inhibits endothelial cell proliferation and tumor angiogenesis. As a result, TIMP-2...", "pubmed_id": 23371049, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["cancer", "cell", "endothelial cell", "lung", "lung cancer", "scid"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptional-profiles-of-timp-2-and-alatimp-2-a", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38408", "geo_id_plat": "E-GEOD-38408_A-AFFY-44", "name": "Transcriptional profiles of TIMP-2 and Ala+TIMP-2 A549 overexpressing cells and in tumor xenografts.", "created": "Jul.12, 2014", "summary": "TIMP-2 is an endogenous angiogenesis inhibitor, i.e. inhibits endothelial cell proliferation and tumor angiogenesis. As a result, TIMP-2 inhibits tumor growth and progression to metastasis. Understanding, therefore, the mechanisms of TIMP-2-mediated tumor growth inhibition would provide further support on the use of TIMP-2 as a novel biological agent for cancer therapy. We used microarray analysis to determine the TIMP-2 and Ala+TIMP-2 transcriptional profiles of A549 cancer cells in order to understand how TIMP-2 inhibits tumor growth and angiogenesis. We overexpressed TIMP-2 and its mutant (does not inhibit MMP) in A549 human lung cancer cells and determined TIMP-2 and Ala+TIMP-2 transcriptional profiles, groups of genes and associated biological functions. We then injected the cells in NOD-SCID mice and RNA from tumors were isolated for further analysis to identify genes that are associated with tumor growth inhibition.", "geo_gse_id": "E-GEOD-38408", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38408/samples/"}