ArrayExpress Uploaderarrayexpress_sidhumantype 2 diabetic adult undergoing leg amputation87maletype 2 diabetic adult undergoing leg amputation77femaletype 2 diabetic adult undergoing leg amputation56maletype 2 diabetic adult undergoing leg amputation76malehealthy adults undergoing elective surgery (abdominoplasty)35femalehealthy adults undergoing elective surgery (abdominoplasty)26malehealthy adults undergoing elective surgery (abdominoplasty)31malehealthy adults undergoing elective surgery (abdominoplasty)84male449904Analysis of ex vivo isolated lymphatic endothelial cells from the dermis of patients to define type 2 diabetes-induced changes. Results...E-GEOD-38396/profile/8773/arrayexpressuploader38dermisdiseaseskinDec.12, 2014FalseE-GEOD-38396_A-AFFY-44dermal-lymphatic-endothelial-cell-response-to-typeDermal lymphatic endothelial cell response to type 2 diabetes [Homo sapiens]Sep.19, 2014Analysis of ex vivo isolated lymphatic endothelial cells from the dermis of patients to define type 2 diabetes-induced changes. Results preveal aberrant dermal lymphangiogenesis and provide insight into its role in the pathogenesis of persistent skin inflammation in type 2 diabetes. The ex vivo dLEC transcriptome reveals a dramatic influence of the T2D environment on multiple molecular and cellular processes, mirroring the phenotypic changes seen in T2D affected skin. The positively and negatively correlated dLEC transcripts directly cohere to prolonged inflammatory periods and reduced infectious resistance of patients´ skin. Further, lymphatic vessels might be involved in tissue remodeling processes during T2D induced skin alterations associated with impaired wound healing and altered dermal architecture. Hence, dermal lymphatic vessels might be directly associated with T2D disease promotion. Global gene expression profile of normal dermal lymphatic endothelial cells (ndLECs) compared to dermal lymphatic endothelial cells derived from type 2 diabetic patients (dLECs).Quadruplicate biological samples were analyzed from human lymphatic endothelial cells (4 x diabetic; 4 x non-diabetic). subsets: 1 disease state set (dLECs), 1 control set (ndLECs)http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38396http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38396/samples/