{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM940846": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "wild type"}}, {"GSM940846": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "wild type"}}, {"GSM940846": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "wild type"}}, {"GSM940849": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "wild type"}}, {"GSM940849": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "wild type"}}, {"GSM940849": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "wild type"}}, {"GSM940852": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "wild type"}}, {"GSM940852": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "wild type"}}, {"GSM940852": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "wild type"}}, {"GSM940855": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM940855": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM940855": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM940858": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM940858": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM940858": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM94086": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM94086": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM94086": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "C57BL6-STAT1<tm1Dlv> (STAT1KO)"}}, {"GSM940864": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940864": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940864": {"TREATMENT": "stimulated for 3 hours with medium alone", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940867": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940867": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940867": {"TREATMENT": "stimulated for 3 hours with IL-6 (20 ng/ml)", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940870": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940870": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}, {"GSM940870": {"TREATMENT": "stimulated for 3 hours with IL-27 (20 ng/ml)", "VARIATION": "Cd4-Cre_C57BL6-STAT3<tm1Dlv> (STAT3KO)"}}], "id": 6911, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Interleukin (IL)-27 is a key immunosuppressive cytokine that counters T helper 17 (Th17) cell-mediated pathology. To identify mechanisms...", "geo_gse_id": "E-GEOD-38375", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 27, "tags": ["cell", "cytokine", "encephalomyelitis", "genome", "interleukin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "interleukin-27-priming-of-t-cells-controls-interle", "geo_id_plat": "E-GEOD-38375_A-AFFY-45", "name": "Interleukin-27 priming of T cells controls Interleukin-17-production in trans via induction of Programmed cell death ligand 1", "created": "Nov.12, 2014", "summary": "Interleukin (IL)-27 is a key immunosuppressive cytokine that counters T helper 17 (Th17) cell-mediated pathology. To identify mechanisms by which IL-27 might exert its immunosuppressive effect, we analyzed genes in T cells rapidly induced by IL-27. We found that IL-27 priming of na\u00efve T cells upregulated expression of programmed death ligand 1 (PD-L1) in a signal transducer and activator of transcription (STAT)1-dependent manner. When co-cultured with na\u00efve CD4+ T cells, IL-27-primed T cells inhibited the differentiation of Th17 cells in trans through a PD-1-PD-L1 interaction. In vivo, co-administration of na\u00efve TCR transgenic T cells (2D2 T cells) with IL-27-primed T cells expressing PD-L1 inhibited the development of Th17 cells and protected from severe autoimmune encephalomyelitis. Thus, these data identify a suppressive activity of IL-27, by which CD4+ T cells can restrict differentiation of Th17 cells in trans. The roles of IL-6 and IL-27 in na\u00efve CD4+ T cells was investigated by comparing global gene expression by Affymetrix Mouse Genome 430 2.0 Arrays. The functional outcome of STAT proteins was further evaluated by profiling gene expression changes between WT and STAT-deficient T cells in na\u00efve CD4+ T cells with specific stimulation. All condition were done in biological triplicate.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38375", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38375/samples/"}