{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 9713, "factors": [{"GSM940450 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940451 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940452 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940453 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940454 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940455 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940456 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940457 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940458 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940459 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940460 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940461 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940462 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940463 1": {"disease state": "systemic lupus erythematosus", "growth condition": "none"}}, {"GSM940464 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940465 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940466 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940467 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940468 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940469 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940470 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940471 1": {"disease state": "rheumatoid arthritis", "growth condition": "none"}}, {"GSM940472 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940473 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940474 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940475 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940476 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940477 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940478 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940479 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940480 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940481 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940482 1": {"disease state": "healthy", "growth condition": "none"}}, {"GSM940483 1": {"disease state": "healthy", "growth condition": "none"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "Many cytokines are involved in the pathogenesis of autoimmune diseases and are recognized as relevant therapeutic targets to attenuate...", "pubmed_id": 22610275, "geo_gse_id": "E-GEOD-38351", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 34, "tags": ["cell", "cytokine", "disease", "peripheral"], "lastmodified": "Jan.17, 2015", "is_default": false, "geo_gds_id": "", "slug": "the-multifaceted-balance-of-tnf-a-and-type-i-ii-in", "geo_id_plat": "E-GEOD-38351_A-AFFY-33", "name": "The multifaceted balance of TNF-a and type I / II interferon responses in SLE and RA: how monocytes manage the impact of cytokines", "created": "Jan.17, 2015", "summary": "Many cytokines are involved in the pathogenesis of autoimmune diseases and are recognized as relevant therapeutic targets to attenuate inflammation, such as TNF\u03b1 in RA and IFN\u03b1/\u03b3 in SLE. To relate the transcriptional imprinting of cytokines in a cell type-specific and disease-specific manner, we generated gene-expression profiles from peripheral monocytes of SLE and RA patients and compared them to in vitro-generated signatures induced by TNF\u03b1, IFN\u03b12a and IFN\u03b3. Monocytes from SLE and RA patients revealed disease-specific gene-expression profiles. In vitro-generated signatures induced by IFN\u03b12a and IFN\u03b3 showed similar profiles that only partially overlapped with those induced by TNF\u03b1. Comparisons between disease-specific and in vitro-generated signatures identified cytokine-regulated genes in SLE and RA with qualitative and quantitative differences. The IFN-responses in SLE and RA were found to be regulated in a STAT1-dependent and STAT1-independent manner, respectively. Similarly, genes recognized as TNF\u03b1-regulated were clearly distinguishable between RA and SLE patients. While the activity of SLE monocytes was mainly driven by IFN, the activity from RA monocytes showed a dominance of TNF\u03b1 that was characterized by STAT1 down-regulation. The responses to specific cytokines were revealed to be disease-dependent and reflected the interplay of cytokines within various inflammatory milieus. This study has demonstrated that monocytes from RA and SLE patients exhibit disease-specific gene-expression profiles, which can be molecularly dissected when compared to in vitro-generated cytokine signatures. The results suggest that an assessment of cytokine-response status in monocytes may be helpful for improvement of diagnosis and selection of the best cytokine target for therapeutic intervention. Expression profiles of human peripheral blood monocytes activated in vivo and stimulated in vitro. Monocytes from patients with SLE and RA and from healthy donors were used for generating disease-specific gene-expression profiles, where these profiles represent in vivo activation of monocytes. In addition, monocytes from healthy donors were stimulated in vitro by cytokines: TNF\u03b1, IFN\u03b12a and IFN\u03b3. Cytokine-specific gene-expression profiles were generated by comparing stimulated monocytes with unstimulated ones. TNF\u03b1-, IFN\u03b12a- and IFN\u03b3 as cytokine-specific gene-expression profiles were compared with RA and SLE, as disease-specific gene-expression profiles.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38351", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38351/samples/"}